Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30415 | 5' | -60.3 | NC_006548.1 | + | 5358 | 1.06 | 0.000271 |
Target: 5'- aCGCCGAGGCUCCUGCAACCCCCGAAAa -3' miRNA: 3'- -GCGGCUCCGAGGACGUUGGGGGCUUU- -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 26316 | 0.76 | 0.063997 |
Target: 5'- gGCCGGGuCUCCUGCAGCCaCuuGAAGa -3' miRNA: 3'- gCGGCUCcGAGGACGUUGG-GggCUUU- -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 31017 | 0.7 | 0.167802 |
Target: 5'- uCGCCcAGGUUUCgGCGACCCUCGGu- -3' miRNA: 3'- -GCGGcUCCGAGGaCGUUGGGGGCUuu -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 17001 | 0.69 | 0.208314 |
Target: 5'- aGCCGGGcGCUCC-GgGACCgCCGAc- -3' miRNA: 3'- gCGGCUC-CGAGGaCgUUGGgGGCUuu -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 34402 | 0.69 | 0.216219 |
Target: 5'- uCGCUGuuGGCUCCUGguACucgggcugucgcguuCCCCGGAu -3' miRNA: 3'- -GCGGCu-CCGAGGACguUG---------------GGGGCUUu -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 36820 | 0.68 | 0.225566 |
Target: 5'- gGCgCGAGGCUCuCUGCGAgcgUCgCCGAGc -3' miRNA: 3'- gCG-GCUCCGAG-GACGUU---GGgGGCUUu -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 31069 | 0.67 | 0.2637 |
Target: 5'- cCGCCuGGcGCUCCgGCAACCCgUGGc- -3' miRNA: 3'- -GCGGcUC-CGAGGaCGUUGGGgGCUuu -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 22097 | 0.67 | 0.270542 |
Target: 5'- uGCCGAuGGCcuUCCUGCAggaGCUgCUGGAAc -3' miRNA: 3'- gCGGCU-CCG--AGGACGU---UGGgGGCUUU- -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 11731 | 0.67 | 0.283935 |
Target: 5'- gCGCCGGGGUUgCgGCGaugacaugacgauGCUCCCGAu- -3' miRNA: 3'- -GCGGCUCCGAgGaCGU-------------UGGGGGCUuu -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 7811 | 0.67 | 0.284654 |
Target: 5'- uGCCGcgagcugcgcaAGGCUCUgcGCGACCUCCGcGAg -3' miRNA: 3'- gCGGC-----------UCCGAGGa-CGUUGGGGGCuUU- -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 11987 | 0.66 | 0.3386 |
Target: 5'- aGCCGAGGa---UGCAGCCCaggaCGAu- -3' miRNA: 3'- gCGGCUCCgaggACGUUGGGg---GCUuu -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 8765 | 0.66 | 0.344385 |
Target: 5'- aCGCCGuuggGGcGCUCCUggcuuuccaugccgGCAGCCaagaCCGAGAc -3' miRNA: 3'- -GCGGC----UC-CGAGGA--------------CGUUGGg---GGCUUU- -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 18301 | 0.66 | 0.346051 |
Target: 5'- uGUCGAGGUUCCUGaagguauCGACCCUggcUGGGAc -3' miRNA: 3'- gCGGCUCCGAGGAC-------GUUGGGG---GCUUU- -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 27594 | 0.66 | 0.346887 |
Target: 5'- gGCCGuaaccGGCgaugCCggcgaacaGCAGCCCCUGGAc -3' miRNA: 3'- gCGGCu----CCGa---GGa-------CGUUGGGGGCUUu -5' |
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30415 | 5' | -60.3 | NC_006548.1 | + | 35246 | 0.66 | 0.355318 |
Target: 5'- uGCCGcccucGGC-CUUGUcuCCCCCGAAc -3' miRNA: 3'- gCGGCu----CCGaGGACGuuGGGGGCUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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