Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30418 | 3' | -52 | NC_006548.1 | + | 37484 | 0.68 | 0.631573 |
Target: 5'- cUGGCACCCgu-UGCCCACCa----- -3' miRNA: 3'- uGCUGUGGGuugACGGGUGGaaauac -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 35057 | 0.66 | 0.767829 |
Target: 5'- uACGGcCGCCC-AUUGCUCGCCUc---- -3' miRNA: 3'- -UGCU-GUGGGuUGACGGGUGGAaauac -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 34138 | 0.67 | 0.723763 |
Target: 5'- --uGCACCCGGCUGUCgGCCg----- -3' miRNA: 3'- ugcUGUGGGUUGACGGgUGGaaauac -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 29275 | 0.69 | 0.608283 |
Target: 5'- uCGACaauACCCGGCUGgCCGCCg----- -3' miRNA: 3'- uGCUG---UGGGUUGACgGGUGGaaauac -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 26290 | 0.66 | 0.767829 |
Target: 5'- cCGACAUCCAGCgcgccGCgCGCUUUUAc- -3' miRNA: 3'- uGCUGUGGGUUGa----CGgGUGGAAAUac -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 24532 | 0.69 | 0.619922 |
Target: 5'- gGCGAUcuggGCCUcgcagaucgAGCUGCCCGCCgacacgGUGa -3' miRNA: 3'- -UGCUG----UGGG---------UUGACGGGUGGaaa---UAC- -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 23727 | 0.67 | 0.70563 |
Target: 5'- cACGAuCACCCAACUgcgagauuuccgcacGCCagcaGCCUaUGUGc -3' miRNA: 3'- -UGCU-GUGGGUUGA---------------CGGg---UGGAaAUAC- -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 22244 | 0.7 | 0.505763 |
Target: 5'- cCGACACCCGcaACgGCaCCACCUUc--- -3' miRNA: 3'- uGCUGUGGGU--UGaCG-GGUGGAAauac -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 21147 | 0.69 | 0.585086 |
Target: 5'- aGCGGCGCCaucGCcGCCCGCCguaucGUGa -3' miRNA: 3'- -UGCUGUGGgu-UGaCGGGUGGaaa--UAC- -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 13304 | 0.68 | 0.678061 |
Target: 5'- uCGGCGCCgGuUUGCCCGCCg----- -3' miRNA: 3'- uGCUGUGGgUuGACGGGUGGaaauac -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 9603 | 0.67 | 0.689593 |
Target: 5'- cGCGGCGCgCCAGgUGUCCAUCg----- -3' miRNA: 3'- -UGCUGUG-GGUUgACGGGUGGaaauac -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 5852 | 1.08 | 0.00151 |
Target: 5'- gACGACACCCAACUGCCCACCUUUAUGc -3' miRNA: 3'- -UGCUGUGGGUUGACGGGUGGAAAUAC- -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 1729 | 0.7 | 0.562064 |
Target: 5'- gGCGGCAgguccaUCCAACUGCUCGCCc----- -3' miRNA: 3'- -UGCUGU------GGGUUGACGGGUGGaaauac -5' |
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30418 | 3' | -52 | NC_006548.1 | + | 1375 | 0.67 | 0.723763 |
Target: 5'- cCGACuuuCUCGAuCUGCCCGCCg----- -3' miRNA: 3'- uGCUGu--GGGUU-GACGGGUGGaaauac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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