Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30419 | 3' | -57.3 | NC_006548.1 | + | 6151 | 1.12 | 0.000208 |
Target: 5'- gUGUGCUGAUCACCCGCAACCCCUGGCg -3' miRNA: 3'- -ACACGACUAGUGGGCGUUGGGGACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 7092 | 0.78 | 0.081154 |
Target: 5'- cGUGCUGAUCACCaggGCcucgucacGCCCCaGGCg -3' miRNA: 3'- aCACGACUAGUGGg--CGu-------UGGGGaCCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 7623 | 0.7 | 0.276558 |
Target: 5'- --cGCUGGUCGgCCGuCAugguuuCCaCCUGGCg -3' miRNA: 3'- acaCGACUAGUgGGC-GUu-----GG-GGACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 8876 | 0.7 | 0.283773 |
Target: 5'- --cGCUGAUCACaCGCua--CCUGGCg -3' miRNA: 3'- acaCGACUAGUGgGCGuuggGGACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 9635 | 0.76 | 0.108464 |
Target: 5'- -aUGCacUGAUCGCgUGC-GCCCCUGGCg -3' miRNA: 3'- acACG--ACUAGUGgGCGuUGGGGACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 12260 | 0.77 | 0.096634 |
Target: 5'- cGUGCUGAU-GCCCGCucGCCCgUcGGCg -3' miRNA: 3'- aCACGACUAgUGGGCGu-UGGGgA-CCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 12396 | 0.75 | 0.125162 |
Target: 5'- --cGCUGGUCACCUaCGACCgUUGGCu -3' miRNA: 3'- acaCGACUAGUGGGcGUUGGgGACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 14558 | 0.66 | 0.469081 |
Target: 5'- --gGCUGAUCGCgUCG-GACUgCUGGCg -3' miRNA: 3'- acaCGACUAGUG-GGCgUUGGgGACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 16315 | 0.66 | 0.489802 |
Target: 5'- gGUGCccaacGAUUcgauCCUGCAGCgCCgUGGCg -3' miRNA: 3'- aCACGa----CUAGu---GGGCGUUG-GGgACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 20871 | 0.66 | 0.45889 |
Target: 5'- --aGCUcGAUCACCgG-AGCCuUCUGGCg -3' miRNA: 3'- acaCGA-CUAGUGGgCgUUGG-GGACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 23527 | 0.71 | 0.249162 |
Target: 5'- --gGCcgGAUgACCCGCuGACCCCgccaGGCg -3' miRNA: 3'- acaCGa-CUAgUGGGCG-UUGGGGa---CCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 26316 | 0.68 | 0.372966 |
Target: 5'- --gGCcgGGUCuCCUGCAGCCacuugaagaCCUGGCg -3' miRNA: 3'- acaCGa-CUAGuGGGCGUUGG---------GGACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 27141 | 0.66 | 0.479386 |
Target: 5'- cGUaCUGG-CugCCGCAGCaUCCUGGg -3' miRNA: 3'- aCAcGACUaGugGGCGUUG-GGGACCg -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 27527 | 0.68 | 0.364115 |
Target: 5'- -cUGCUGuUCGCCgGCAucGCCgguuacggCCUGGCc -3' miRNA: 3'- acACGACuAGUGGgCGU--UGG--------GGACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 27816 | 0.66 | 0.469081 |
Target: 5'- --cGCUGcaccUCACCCGCcaggaAACCaaUGGCa -3' miRNA: 3'- acaCGACu---AGUGGGCG-----UUGGggACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 28341 | 0.78 | 0.081154 |
Target: 5'- --cGUUGAcgaCGCCCGCAGCCCCUuuGGCc -3' miRNA: 3'- acaCGACUa--GUGGGCGUUGGGGA--CCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 30628 | 0.72 | 0.194114 |
Target: 5'- cGUGCUGAccUUgacgaccagccagaACCUGCGGCCgUUGGCg -3' miRNA: 3'- aCACGACU--AG--------------UGGGCGUUGGgGACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 30751 | 0.68 | 0.372966 |
Target: 5'- --aGCUGcuAUC-UCCGCGAUCCCgccgGGCg -3' miRNA: 3'- acaCGAC--UAGuGGGCGUUGGGGa---CCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 35161 | 0.72 | 0.216943 |
Target: 5'- aGUGC-GAUCACCUGCcuugaucucgaccAGcucauccaacggcCCCCUGGCa -3' miRNA: 3'- aCACGaCUAGUGGGCG-------------UU-------------GGGGACCG- -5' |
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30419 | 3' | -57.3 | NC_006548.1 | + | 37760 | 0.71 | 0.224047 |
Target: 5'- --aGCUGAUCugUCGuCGGCcucaccgaaCCCUGGCa -3' miRNA: 3'- acaCGACUAGugGGC-GUUG---------GGGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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