Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30420 | 3' | -56.5 | NC_006548.1 | + | 33871 | 0.67 | 0.523094 |
Target: 5'- cGCGCGGCCg--GUGAGcGACGAuGGAc- -3' miRNA: 3'- -CGCGCUGGaggCGCUC-CUGUU-CCUuc -5' |
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30420 | 3' | -56.5 | NC_006548.1 | + | 29435 | 0.69 | 0.38425 |
Target: 5'- cCGCGGCCUCCuGC-AGcGACAGGGu-- -3' miRNA: 3'- cGCGCUGGAGG-CGcUC-CUGUUCCuuc -5' |
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30420 | 3' | -56.5 | NC_006548.1 | + | 25356 | 0.68 | 0.440863 |
Target: 5'- uGCGCGACCagCgaaugGUGGGcGGCAAGGAc- -3' miRNA: 3'- -CGCGCUGGagG-----CGCUC-CUGUUCCUuc -5' |
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30420 | 3' | -56.5 | NC_006548.1 | + | 20490 | 0.72 | 0.245272 |
Target: 5'- cGCGCGuCCUCCucguCGAGGACAacugccauuguGGGAu- -3' miRNA: 3'- -CGCGCuGGAGGc---GCUCCUGU-----------UCCUuc -5' |
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30420 | 3' | -56.5 | NC_006548.1 | + | 19156 | 0.67 | 0.523094 |
Target: 5'- aGCG-GGCUUCUGC-AGGAUAucGGGGAGc -3' miRNA: 3'- -CGCgCUGGAGGCGcUCCUGU--UCCUUC- -5' |
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30420 | 3' | -56.5 | NC_006548.1 | + | 16923 | 0.66 | 0.530587 |
Target: 5'- -aGCGGCCgaCCGcCGuGGGCAuaccgaacuuguccAGGAAGa -3' miRNA: 3'- cgCGCUGGa-GGC-GCuCCUGU--------------UCCUUC- -5' |
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30420 | 3' | -56.5 | NC_006548.1 | + | 15864 | 0.67 | 0.512461 |
Target: 5'- gGCuGUGACCUucaccCCGCGAGua-AAGGAGGc -3' miRNA: 3'- -CG-CGCUGGA-----GGCGCUCcugUUCCUUC- -5' |
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30420 | 3' | -56.5 | NC_006548.1 | + | 13159 | 0.68 | 0.450752 |
Target: 5'- cGCGCGACCUgCUGCuuguacuGGACcAGGGc- -3' miRNA: 3'- -CGCGCUGGA-GGCGcu-----CCUGuUCCUuc -5' |
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30420 | 3' | -56.5 | NC_006548.1 | + | 7789 | 1.09 | 0.000492 |
Target: 5'- uGCGCGACCUCCGCGAGGACAAGGAAGc -3' miRNA: 3'- -CGCGCUGGAGGCGCUCCUGUUCCUUC- -5' |
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30420 | 3' | -56.5 | NC_006548.1 | + | 3991 | 0.72 | 0.272085 |
Target: 5'- uGCGCGAUggUUUCGCGaAGGACcAGGAGc -3' miRNA: 3'- -CGCGCUG--GAGGCGC-UCCUGuUCCUUc -5' |
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30420 | 3' | -56.5 | NC_006548.1 | + | 3145 | 0.66 | 0.555468 |
Target: 5'- aGCGCGACCgCCuuGUGcAGGAgAucguugccaagGGGAAGg -3' miRNA: 3'- -CGCGCUGGaGG--CGC-UCCUgU-----------UCCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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