Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30421 | 3' | -56.6 | NC_006548.1 | + | 3890 | 0.67 | 0.48126 |
Target: 5'- gGCCACuucuccaagcugCAUG-CGGAGCuGCAGUGCaUGGc -3' miRNA: 3'- -CGGUG------------GUACuGCCUCG-UGUCACG-GCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 6686 | 0.7 | 0.315645 |
Target: 5'- cGCCGagaGUGGCGaGAacuucuGCACGGUGCUGAu -3' miRNA: 3'- -CGGUgg-UACUGC-CU------CGUGUCACGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 7580 | 0.69 | 0.365778 |
Target: 5'- uUCGCCAaguUGGCcgagcaggccgaGGAGCAaGGUGCCGAc -3' miRNA: 3'- cGGUGGU---ACUG------------CCUCGUgUCACGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 8220 | 1.11 | 0.000313 |
Target: 5'- cGCCACCAUGACGGAGCACAGUGCCGAg -3' miRNA: 3'- -CGGUGGUACUGCCUCGUGUCACGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 16012 | 0.68 | 0.440686 |
Target: 5'- uCCGCUucacuGCGGAGCGCAcUGCUGAc -3' miRNA: 3'- cGGUGGuac--UGCCUCGUGUcACGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 16173 | 0.66 | 0.54525 |
Target: 5'- aUCAUCGUGccgcACGGcuucGGCGCGGUGCgCGGu -3' miRNA: 3'- cGGUGGUAC----UGCC----UCGUGUCACG-GCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 16974 | 0.67 | 0.470942 |
Target: 5'- cGCCGgCAUGcagGCGGAGCuGCuGgaaGCCGGg -3' miRNA: 3'- -CGGUgGUAC---UGCCUCG-UGuCa--CGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 17027 | 0.66 | 0.502218 |
Target: 5'- cGCCugCAUGcCGGcgGGCAUgaugauUGCCGAa -3' miRNA: 3'- -CGGugGUACuGCC--UCGUGuc----ACGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 17876 | 0.66 | 0.534369 |
Target: 5'- gGCCAuCCGUGACGGcaugacccuGGCGgAGUuCCGc -3' miRNA: 3'- -CGGU-GGUACUGCC---------UCGUgUCAcGGCu -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 20359 | 0.67 | 0.45065 |
Target: 5'- cGCCugCGUGACuucuucgucGAGCGCgaaGGcGCCGAa -3' miRNA: 3'- -CGGugGUACUGc--------CUCGUG---UCaCGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 20622 | 0.68 | 0.421139 |
Target: 5'- -gCACCGUcgGAUGGGGUgACGGUGCCc- -3' miRNA: 3'- cgGUGGUA--CUGCCUCG-UGUCACGGcu -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 21052 | 0.7 | 0.323628 |
Target: 5'- cGCUGCaAUGGCGuGAGCAC--UGCCGAa -3' miRNA: 3'- -CGGUGgUACUGC-CUCGUGucACGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 22072 | 0.71 | 0.270876 |
Target: 5'- gGCUcCCucggcGACGaGGGCAUGGUGCCGAu -3' miRNA: 3'- -CGGuGGua---CUGC-CUCGUGUCACGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 23598 | 0.66 | 0.535453 |
Target: 5'- cCCACCGccUGGCGGGGUcaGCGGgucauccggcccgagGCUGAa -3' miRNA: 3'- cGGUGGU--ACUGCCUCG--UGUCa--------------CGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 23972 | 0.69 | 0.365778 |
Target: 5'- cGUCACCGaGuACGGGGCGCAG-GUCa- -3' miRNA: 3'- -CGGUGGUaC-UGCCUCGUGUCaCGGcu -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 24155 | 0.66 | 0.534369 |
Target: 5'- cGCCACacgcauuccggCAUGACGuaccaGGC-CGGUGCUGAa -3' miRNA: 3'- -CGGUG-----------GUACUGCc----UCGuGUCACGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 26525 | 0.66 | 0.522489 |
Target: 5'- cCCGCCGUacugggaGACGGAGgGCuauggcGUGCCa- -3' miRNA: 3'- cGGUGGUA-------CUGCCUCgUGu-----CACGGcu -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 27779 | 0.66 | 0.556202 |
Target: 5'- cGCCACCA-GGCGGgaauaggcuuGGC-UGGUGuuGAg -3' miRNA: 3'- -CGGUGGUaCUGCC----------UCGuGUCACggCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 27885 | 0.66 | 0.54525 |
Target: 5'- uGCCAUUGguuuccUGGCGGgugaggugcAGCGCAGUggugGCCGAg -3' miRNA: 3'- -CGGUGGU------ACUGCC---------UCGUGUCA----CGGCU- -5' |
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30421 | 3' | -56.6 | NC_006548.1 | + | 28954 | 0.66 | 0.502218 |
Target: 5'- gGCCGCCucGACGGuuuGGC-CGGUGgcCCGAa -3' miRNA: 3'- -CGGUGGuaCUGCC---UCGuGUCAC--GGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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