miRNA: 3'- -CGGUGgUACUGC-CUCGUGucACGGCU- -5'
miRNA: 3'- -CGGUgg-UACUGC-CU------CGUGUCACGGCU- -5'
miRNA: 3'- -CGGuGGua---CUGC-CUCGUGUCACGGCU- -5'
miRNA: 3'- -CGGUGGUACUGCCUCGUGUCACGGCU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30421 | 3' | -56.6 | NC_006548.1 | + | 21052 | 0.7 | 0.323628 |
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Target: 5'- cGCUGCaAUGGCGuGAGCAC--UGCCGAa -3' miRNA: 3'- -CGGUGgUACUGC-CUCGUGucACGGCU- -5' |
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| 30421 | 3' | -56.6 | NC_006548.1 | + | 6686 | 0.7 | 0.315645 |
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Target: 5'- cGCCGagaGUGGCGaGAacuucuGCACGGUGCUGAu -3' miRNA: 3'- -CGGUgg-UACUGC-CU------CGUGUCACGGCU- -5' |
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| 30421 | 3' | -56.6 | NC_006548.1 | + | 22072 | 0.71 | 0.270876 |
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Target: 5'- gGCUcCCucggcGACGaGGGCAUGGUGCCGAu -3' miRNA: 3'- -CGGuGGua---CUGC-CUCGUGUCACGGCU- -5' |
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| 30421 | 3' | -56.6 | NC_006548.1 | + | 8220 | 1.11 | 0.000313 |
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Target: 5'- cGCCACCAUGACGGAGCACAGUGCCGAg -3' miRNA: 3'- -CGGUGGUACUGCCUCGUGUCACGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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