miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30422 5' -57 NC_006548.1 + 34391 0.66 0.50007
Target:  5'- uGGGcggcaccUCGCUGuuGGCUCC-UGGUaCUcGGg -3'
miRNA:   3'- -CCC-------AGCGACggCCGAGGuACUA-GA-CU- -5'
30422 5' -57 NC_006548.1 + 21771 0.67 0.43987
Target:  5'- cGGU-GCUGCCGGUUUCGUcGGUggcgCUGAa -3'
miRNA:   3'- cCCAgCGACGGCCGAGGUA-CUA----GACU- -5'
30422 5' -57 NC_006548.1 + 32774 0.68 0.374133
Target:  5'- uGGG-CGCUGCCGGUguggccggacaUCCAgcugCUGGc -3'
miRNA:   3'- -CCCaGCGACGGCCG-----------AGGUacuaGACU- -5'
30422 5' -57 NC_006548.1 + 32088 0.69 0.3653
Target:  5'- cGGUCGUUGCCGGCaucaCCccGAacaucuccgcUCUGGg -3'
miRNA:   3'- cCCAGCGACGGCCGa---GGuaCU----------AGACU- -5'
30422 5' -57 NC_006548.1 + 21992 0.72 0.207928
Target:  5'- cGGGUUGCUgGUCGGaucaCUCCAcUGAUCUGc -3'
miRNA:   3'- -CCCAGCGA-CGGCC----GAGGU-ACUAGACu -5'
30422 5' -57 NC_006548.1 + 8334 1.11 0.000284
Target:  5'- cGGGUCGCUGCCGGCUCCAUGAUCUGAg -3'
miRNA:   3'- -CCCAGCGACGGCCGAGGUACUAGACU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.