Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30422 | 5' | -57 | NC_006548.1 | + | 34391 | 0.66 | 0.50007 |
Target: 5'- uGGGcggcaccUCGCUGuuGGCUCC-UGGUaCUcGGg -3' miRNA: 3'- -CCC-------AGCGACggCCGAGGuACUA-GA-CU- -5' |
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30422 | 5' | -57 | NC_006548.1 | + | 21771 | 0.67 | 0.43987 |
Target: 5'- cGGU-GCUGCCGGUUUCGUcGGUggcgCUGAa -3' miRNA: 3'- cCCAgCGACGGCCGAGGUA-CUA----GACU- -5' |
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30422 | 5' | -57 | NC_006548.1 | + | 32774 | 0.68 | 0.374133 |
Target: 5'- uGGG-CGCUGCCGGUguggccggacaUCCAgcugCUGGc -3' miRNA: 3'- -CCCaGCGACGGCCG-----------AGGUacuaGACU- -5' |
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30422 | 5' | -57 | NC_006548.1 | + | 32088 | 0.69 | 0.3653 |
Target: 5'- cGGUCGUUGCCGGCaucaCCccGAacaucuccgcUCUGGg -3' miRNA: 3'- cCCAGCGACGGCCGa---GGuaCU----------AGACU- -5' |
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30422 | 5' | -57 | NC_006548.1 | + | 21992 | 0.72 | 0.207928 |
Target: 5'- cGGGUUGCUgGUCGGaucaCUCCAcUGAUCUGc -3' miRNA: 3'- -CCCAGCGA-CGGCC----GAGGU-ACUAGACu -5' |
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30422 | 5' | -57 | NC_006548.1 | + | 8334 | 1.11 | 0.000284 |
Target: 5'- cGGGUCGCUGCCGGCUCCAUGAUCUGAg -3' miRNA: 3'- -CCCAGCGACGGCCGAGGUACUAGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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