Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30426 | 5' | -55.4 | NC_006548.1 | + | 28883 | 0.66 | 0.630018 |
Target: 5'- aCCUUCGggccaccGGCCaaaccgucgAgGCGGCcGGCGCc -3' miRNA: 3'- -GGAAGCaa-----CCGGg--------UgCGUCGuUCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 9753 | 0.66 | 0.630018 |
Target: 5'- gCUUUgaGUUGGCCauugagaACGCGGUAgacgucguuacgGGCGUa -3' miRNA: 3'- gGAAG--CAACCGGg------UGCGUCGU------------UCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 1180 | 0.66 | 0.618748 |
Target: 5'- aCCUUCG--GGUUCACGCcaggAGCcGGCuGCa -3' miRNA: 3'- -GGAAGCaaCCGGGUGCG----UCGuUCG-CG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 37477 | 0.66 | 0.618748 |
Target: 5'- ---cCGUU-GCCCAC-CAGCGAGCuagGCg -3' miRNA: 3'- ggaaGCAAcCGGGUGcGUCGUUCG---CG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 13555 | 0.66 | 0.617622 |
Target: 5'- aCCUUCcucgGuGCCUGCGCgggcggcGGCAAGCuGCu -3' miRNA: 3'- -GGAAGcaa-C-CGGGUGCG-------UCGUUCG-CG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 18560 | 0.66 | 0.607491 |
Target: 5'- aCCgUUGagGGCaugaGCGCAGCuGGCGUg -3' miRNA: 3'- -GGaAGCaaCCGgg--UGCGUCGuUCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 25983 | 0.66 | 0.596257 |
Target: 5'- cCCUcagaUgGUUaaucGCCC-CGCAGCGGGgGCa -3' miRNA: 3'- -GGA----AgCAAc---CGGGuGCGUCGUUCgCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 32866 | 0.66 | 0.585054 |
Target: 5'- uUUUCGUgcugggGGCCuUGCGCuGCu-GCGCg -3' miRNA: 3'- gGAAGCAa-----CCGG-GUGCGuCGuuCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 2930 | 0.66 | 0.585054 |
Target: 5'- gCCUUCGacgaGcGCCUGCagGCGGCcaAGGCGCu -3' miRNA: 3'- -GGAAGCaa--C-CGGGUG--CGUCG--UUCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 33281 | 0.66 | 0.573892 |
Target: 5'- gCUUcCGUUGGCagaccuccggCCGCGaggAGCAugcGGCGCu -3' miRNA: 3'- gGAA-GCAACCG----------GGUGCg--UCGU---UCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 15755 | 0.67 | 0.56278 |
Target: 5'- uCCUc---UGGCCCugguuccaGCGCuGUGAGCGCa -3' miRNA: 3'- -GGAagcaACCGGG--------UGCGuCGUUCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 23945 | 0.67 | 0.56278 |
Target: 5'- cCCUgaUCGcUGGacaCCGCccgGCcuGGCAGGCGCu -3' miRNA: 3'- -GGA--AGCaACCg--GGUG---CG--UCGUUCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 36590 | 0.67 | 0.551727 |
Target: 5'- gCCg-CGgccGGCaCCGCGguGCucGCGCg -3' miRNA: 3'- -GGaaGCaa-CCG-GGUGCguCGuuCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 34033 | 0.67 | 0.551727 |
Target: 5'- gCCUUCGacGGCCgaGCGCcacgguacAGCu-GCGCa -3' miRNA: 3'- -GGAAGCaaCCGGg-UGCG--------UCGuuCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 13109 | 0.67 | 0.549525 |
Target: 5'- gCgUUCGUUGGUgUGCuGCAGCugcagguugagcAGCGCa -3' miRNA: 3'- -GgAAGCAACCGgGUG-CGUCGu-----------UCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 35939 | 0.67 | 0.540741 |
Target: 5'- aCCggcgaGUUGGCCgGCaggGUGGCGGcGCGCg -3' miRNA: 3'- -GGaag--CAACCGGgUG---CGUCGUU-CGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 19249 | 0.67 | 0.540741 |
Target: 5'- cCCUugUCGccGGCCUACcuGCGGCGcaAGCGa -3' miRNA: 3'- -GGA--AGCaaCCGGGUG--CGUCGU--UCGCg -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 3589 | 0.67 | 0.529829 |
Target: 5'- gCUUCGUcGGCguagCAgGUAGCAgccAGCGCc -3' miRNA: 3'- gGAAGCAaCCGg---GUgCGUCGU---UCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 24305 | 0.67 | 0.518999 |
Target: 5'- uCCUUCcagGGCCggguCugGgcCGGCGAGCGCc -3' miRNA: 3'- -GGAAGcaaCCGG----GugC--GUCGUUCGCG- -5' |
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30426 | 5' | -55.4 | NC_006548.1 | + | 12926 | 0.67 | 0.518999 |
Target: 5'- ---aCGUucUGGCUCGCGCugAGCGcggugcugugcGGCGCa -3' miRNA: 3'- ggaaGCA--ACCGGGUGCG--UCGU-----------UCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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