Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30427 | 3' | -48.8 | NC_006548.1 | + | 30403 | 0.66 | 0.889534 |
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Target: 5'- aUGGCGUcgGUgccgcccaGGCCAGggGCGc---- -3' miRNA: 3'- -ACCGCA--UAag------UCGGUCuuCGCuuaau -5' |
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| 30427 | 3' | -48.8 | NC_006548.1 | + | 2695 | 0.67 | 0.872958 |
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Target: 5'- aGGCGUAcgugCGuauCCAGGAGCGggUc- -3' miRNA: 3'- aCCGCAUaa--GUc--GGUCUUCGCuuAau -5' |
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| 30427 | 3' | -48.8 | NC_006548.1 | + | 5428 | 0.67 | 0.845873 |
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Target: 5'- -aGCGauUUCGGCCAGAGGCcguGAUUGa -3' miRNA: 3'- acCGCauAAGUCGGUCUUCGc--UUAAU- -5' |
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| 30427 | 3' | -48.8 | NC_006548.1 | + | 30161 | 0.68 | 0.836291 |
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Target: 5'- cGGCuUAgugUCAGCCAGAAGUu----- -3' miRNA: 3'- aCCGcAUa--AGUCGGUCUUCGcuuaau -5' |
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| 30427 | 3' | -48.8 | NC_006548.1 | + | 16421 | 0.68 | 0.795514 |
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Target: 5'- uUGGcCGUAgUCAGCCGcGAGUGGAa-- -3' miRNA: 3'- -ACC-GCAUaAGUCGGUcUUCGCUUaau -5' |
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| 30427 | 3' | -48.8 | NC_006548.1 | + | 17066 | 0.69 | 0.762736 |
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Target: 5'- aGGuCGggcUCGGCCAGGuGGCGAGUa- -3' miRNA: 3'- aCC-GCauaAGUCGGUCU-UCGCUUAau -5' |
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| 30427 | 3' | -48.8 | NC_006548.1 | + | 19463 | 0.69 | 0.751468 |
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Target: 5'- cUGGCGaagUCGGCCGGGAGUucGUg- -3' miRNA: 3'- -ACCGCauaAGUCGGUCUUCGcuUAau -5' |
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| 30427 | 3' | -48.8 | NC_006548.1 | + | 36059 | 0.69 | 0.751468 |
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Target: 5'- -uGCGUucgCAGCCGGAAGCGc---- -3' miRNA: 3'- acCGCAuaaGUCGGUCUUCGCuuaau -5' |
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| 30427 | 3' | -48.8 | NC_006548.1 | + | 22901 | 0.71 | 0.645296 |
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Target: 5'- cUGGCG-AUUCAGUCgcgcGGcGGCGAGUUGg -3' miRNA: 3'- -ACCGCaUAAGUCGG----UCuUCGCUUAAU- -5' |
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| 30427 | 3' | -48.8 | NC_006548.1 | + | 10772 | 1.08 | 0.002741 |
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Target: 5'- uUGGCGUAUUCAGCCAGAAGCGAAUUAg -3' miRNA: 3'- -ACCGCAUAAGUCGGUCUUCGCUUAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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