Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30428 | 3' | -58.6 | NC_006548.1 | + | 18331 | 0.66 | 0.47355 |
Target: 5'- -cCCGGUaGGUcaGCCGUCGCaucugGCugCUCg -3' miRNA: 3'- cuGGCUA-CCAa-CGGCAGCG-----CGugGGG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 4326 | 0.66 | 0.461527 |
Target: 5'- -cCCGAugccUGGaUGCCGUCaacaacgccaccCGCGCCCUg -3' miRNA: 3'- cuGGCU----ACCaACGGCAGc-----------GCGUGGGG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 20834 | 0.66 | 0.453602 |
Target: 5'- uGCUGGUGaGUcUGcCCGUCGa-CACCCCc -3' miRNA: 3'- cUGGCUAC-CA-AC-GGCAGCgcGUGGGG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 9074 | 0.66 | 0.43412 |
Target: 5'- aGACC--UGGgUGCCG-CGCuCGCCCUg -3' miRNA: 3'- -CUGGcuACCaACGGCaGCGcGUGGGG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 34425 | 0.66 | 0.43124 |
Target: 5'- gGACCaGgcGGUUGcCCGUguguugcucgaugcCGCGCugCUCa -3' miRNA: 3'- -CUGG-CuaCCAAC-GGCA--------------GCGCGugGGG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 21402 | 0.66 | 0.424562 |
Target: 5'- cGCCccUGGgcgucgGCCGUCaGCGCAUCaCCg -3' miRNA: 3'- cUGGcuACCaa----CGGCAG-CGCGUGG-GG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 13961 | 0.67 | 0.393032 |
Target: 5'- cGGCCGGUGGgcccuacgagggGCCGguccucucggUCGCacagagcgaGUACCCCg -3' miRNA: 3'- -CUGGCUACCaa----------CGGC----------AGCG---------CGUGGGG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 21575 | 0.67 | 0.37873 |
Target: 5'- -uCCGAcgaGGUucUGCCG-CGCGUACCUg -3' miRNA: 3'- cuGGCUa--CCA--ACGGCaGCGCGUGGGg -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 22312 | 0.67 | 0.37873 |
Target: 5'- aGGCCGAaGGUggUGCCGUUGCGggUgUCg -3' miRNA: 3'- -CUGGCUaCCA--ACGGCAGCGCguGgGG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 23221 | 0.67 | 0.37873 |
Target: 5'- aGGCCGA-GGUguaGCCGcUUGCGacaACUCCu -3' miRNA: 3'- -CUGGCUaCCAa--CGGC-AGCGCg--UGGGG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 1925 | 0.68 | 0.35287 |
Target: 5'- -cCCGAuccccUGGUgcaGCCG-CGCGCGCuugCCCg -3' miRNA: 3'- cuGGCU-----ACCAa--CGGCaGCGCGUG---GGG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 34409 | 0.69 | 0.30499 |
Target: 5'- uGGCUccUGGUacucggGCUGUCGCGUuCCCCg -3' miRNA: 3'- -CUGGcuACCAa-----CGGCAGCGCGuGGGG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 36033 | 0.71 | 0.230472 |
Target: 5'- aGCCGAcgucgacacGUUGCaGUCGCGCGCCgCCa -3' miRNA: 3'- cUGGCUac-------CAACGgCAGCGCGUGG-GG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 29209 | 0.72 | 0.181471 |
Target: 5'- aGACCGAUGcaggucuagcaGUUGCUGcCGCGCGgCCa -3' miRNA: 3'- -CUGGCUAC-----------CAACGGCaGCGCGUgGGg -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 20127 | 0.73 | 0.149966 |
Target: 5'- cGGCauaGAgca-UGCCGUCGCGCACCuCCa -3' miRNA: 3'- -CUGg--CUaccaACGGCAGCGCGUGG-GG- -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 13347 | 0.76 | 0.101448 |
Target: 5'- cGCCGAgggcgGuGUUGaaGUCGCGCACCCa -3' miRNA: 3'- cUGGCUa----C-CAACggCAGCGCGUGGGg -5' |
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30428 | 3' | -58.6 | NC_006548.1 | + | 11136 | 1.1 | 0.000249 |
Target: 5'- cGACCGAUGGUUGCCGUCGCGCACCCCc -3' miRNA: 3'- -CUGGCUACCAACGGCAGCGCGUGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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