Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30429 | 3' | -54.6 | NC_006548.1 | + | 34061 | 0.66 | 0.665689 |
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Target: 5'- gGAUCGA-CgAGgugaacUGGCCGGCCgacaGCCGg -3' miRNA: 3'- gCUAGCUaGgUU------AUCGGCCGG----UGGCg -5' |
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| 30429 | 3' | -54.6 | NC_006548.1 | + | 22361 | 0.66 | 0.676887 |
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Target: 5'- -aGUCGGUCaaggaaCUGGUCACCGCa -3' miRNA: 3'- gcUAGCUAGguuaucGGCCGGUGGCG- -5' |
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| 30429 | 3' | -54.6 | NC_006548.1 | + | 14340 | 0.66 | 0.68804 |
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Target: 5'- gCGAUCagcucGUCCAGggucauCCGGCCAUCGg -3' miRNA: 3'- -GCUAGc----UAGGUUauc---GGCCGGUGGCg -5' |
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| 30429 | 3' | -54.6 | NC_006548.1 | + | 13638 | 0.66 | 0.68804 |
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Target: 5'- uGGUUGAg-CAGcAGCUuGCCGCCGCc -3' miRNA: 3'- gCUAGCUagGUUaUCGGcCGGUGGCG- -5' |
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| 30429 | 3' | -54.6 | NC_006548.1 | + | 37095 | 0.66 | 0.68804 |
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Target: 5'- gCGGguaGGUCCAGgucguGCCGGUCaaGCCGg -3' miRNA: 3'- -GCUag-CUAGGUUau---CGGCCGG--UGGCg -5' |
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| 30429 | 3' | -54.6 | NC_006548.1 | + | 29755 | 0.65 | 0.694706 |
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Target: 5'- uGAUCGAggacagccagccgCCAcUA-CCGGCaGCCGCa -3' miRNA: 3'- gCUAGCUa------------GGUuAUcGGCCGgUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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