Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30429 | 3' | -54.6 | NC_006548.1 | + | 5568 | 0.7 | 0.408713 |
Target: 5'- aCGcgCaggCCAGgccgGGCCGGCUugCGCa -3' miRNA: 3'- -GCuaGcuaGGUUa---UCGGCCGGugGCG- -5' |
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30429 | 3' | -54.6 | NC_006548.1 | + | 28969 | 0.71 | 0.362922 |
Target: 5'- --cUCGAUCUcGgcGCCGGCCGCCu- -3' miRNA: 3'- gcuAGCUAGGuUauCGGCCGGUGGcg -5' |
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30429 | 3' | -54.6 | NC_006548.1 | + | 36656 | 0.73 | 0.305026 |
Target: 5'- cCGcgCGAgcaCCGcgGuGCCGGCCGCgGCg -3' miRNA: 3'- -GCuaGCUa--GGUuaU-CGGCCGGUGgCG- -5' |
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30429 | 3' | -54.6 | NC_006548.1 | + | 24248 | 0.73 | 0.268184 |
Target: 5'- gCGGUCGAaacCCAGgacgCGGCCACCGCc -3' miRNA: 3'- -GCUAGCUa--GGUUaucgGCCGGUGGCG- -5' |
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30429 | 3' | -54.6 | NC_006548.1 | + | 32373 | 0.74 | 0.235019 |
Target: 5'- aGAUCGAUcgcgaggacgCCGAUggcaucagccuGGCCGGCCAgCCGUc -3' miRNA: 3'- gCUAGCUA----------GGUUA-----------UCGGCCGGU-GGCG- -5' |
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30429 | 3' | -54.6 | NC_006548.1 | + | 11881 | 1.13 | 0.000445 |
Target: 5'- gCGAUCGAUCCAAUAGCCGGCCACCGCg -3' miRNA: 3'- -GCUAGCUAGGUUAUCGGCCGGUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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