Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30429 | 3' | -54.6 | NC_006548.1 | + | 33757 | 0.69 | 0.51017 |
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Target: 5'- gCGGUCGGUcgCCGAUGGCCaGGCgAUUaGCu -3' miRNA: 3'- -GCUAGCUA--GGUUAUCGG-CCGgUGG-CG- -5' |
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| 30429 | 3' | -54.6 | NC_006548.1 | + | 34061 | 0.66 | 0.665689 |
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Target: 5'- gGAUCGA-CgAGgugaacUGGCCGGCCgacaGCCGg -3' miRNA: 3'- gCUAGCUaGgUU------AUCGGCCGG----UGGCg -5' |
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| 30429 | 3' | -54.6 | NC_006548.1 | + | 34730 | 0.66 | 0.643207 |
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Target: 5'- gGAUCG-UCCucgGGaCCGGUCACgaGCg -3' miRNA: 3'- gCUAGCuAGGuuaUC-GGCCGGUGg-CG- -5' |
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| 30429 | 3' | -54.6 | NC_006548.1 | + | 36576 | 0.68 | 0.531758 |
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Target: 5'- uCGAcgCGAUCaacgccgcGGCCGGC-ACCGCg -3' miRNA: 3'- -GCUa-GCUAGguua----UCGGCCGgUGGCG- -5' |
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| 30429 | 3' | -54.6 | NC_006548.1 | + | 36656 | 0.73 | 0.305026 |
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Target: 5'- cCGcgCGAgcaCCGcgGuGCCGGCCGCgGCg -3' miRNA: 3'- -GCuaGCUa--GGUuaU-CGGCCGGUGgCG- -5' |
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| 30429 | 3' | -54.6 | NC_006548.1 | + | 37095 | 0.66 | 0.68804 |
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Target: 5'- gCGGguaGGUCCAGgucguGCCGGUCaaGCCGg -3' miRNA: 3'- -GCUag-CUAGGUUau---CGGCCGG--UGGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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