Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30429 | 5' | -53.6 | NC_006548.1 | + | 2823 | 0.66 | 0.699149 |
Target: 5'- cCGCguGGACCaAGCUgggaacauccGUAccGGCAGCGu -3' miRNA: 3'- aGUGguCCUGG-UCGAa---------CAU--CCGUUGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 37389 | 0.66 | 0.692353 |
Target: 5'- gUCACCGGcagcggcucaaaucaGAaccgcCUAGCUcgcugGUGGGCAACGg -3' miRNA: 3'- -AGUGGUC---------------CU-----GGUCGAa----CAUCCGUUGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 29176 | 0.66 | 0.68781 |
Target: 5'- -gGCCAGuucgcugacgaGACCGGCcUGcUGGGCGGCc -3' miRNA: 3'- agUGGUC-----------CUGGUCGaAC-AUCCGUUGc -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 38195 | 0.66 | 0.68781 |
Target: 5'- cUCACCgAGGACCAGCgcgaauGCAuCGa -3' miRNA: 3'- -AGUGG-UCCUGGUCGaacaucCGUuGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 9882 | 0.66 | 0.654641 |
Target: 5'- cCGCCAGG-CCGGCaaaaccuucaccgaGUGGGCAAa- -3' miRNA: 3'- aGUGGUCCuGGUCGaa------------CAUCCGUUgc -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 30754 | 0.67 | 0.618979 |
Target: 5'- gUCGCUAacgucGGACCAGCggGUu-GCAACGu -3' miRNA: 3'- -AGUGGU-----CCUGGUCGaaCAucCGUUGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 20094 | 0.67 | 0.614379 |
Target: 5'- gCACCAGGugguGCCGGaguucgccgAGGCGGCGa -3' miRNA: 3'- aGUGGUCC----UGGUCgaaca----UCCGUUGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 35014 | 0.67 | 0.607485 |
Target: 5'- -gGCgCAGGGCCAGCUcGUcaccGGUAGCc -3' miRNA: 3'- agUG-GUCCUGGUCGAaCAu---CCGUUGc -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 1192 | 0.68 | 0.573183 |
Target: 5'- aCGCCAGGAgCCGGCUgcaaagccgGUacGGGCGcUGg -3' miRNA: 3'- aGUGGUCCU-GGUCGAa--------CA--UCCGUuGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 7099 | 0.68 | 0.573183 |
Target: 5'- aUCACCAGGGCCucgucacGCccc-AGGCGGCu -3' miRNA: 3'- -AGUGGUCCUGGu------CGaacaUCCGUUGc -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 29673 | 0.68 | 0.561842 |
Target: 5'- gCGgCAGGugCGGCUgccgGUAguGGCGGCu -3' miRNA: 3'- aGUgGUCCugGUCGAa---CAU--CCGUUGc -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 35441 | 0.68 | 0.550563 |
Target: 5'- -gGCCGGcGACCAGg-UGaAGGCGAUGa -3' miRNA: 3'- agUGGUC-CUGGUCgaACaUCCGUUGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 12592 | 0.69 | 0.495379 |
Target: 5'- -aACCAGG-CCAGCUgGUcGGCGcCGg -3' miRNA: 3'- agUGGUCCuGGUCGAaCAuCCGUuGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 5575 | 0.69 | 0.493222 |
Target: 5'- -gGCCAGGccggGCCGGCUUGcgcaacugguuGGCGAUGg -3' miRNA: 3'- agUGGUCC----UGGUCGAACau---------CCGUUGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 2444 | 0.69 | 0.474005 |
Target: 5'- aUCuCCAGGuuguaggcuuccAUCAGUUUGUGGGCAugACGc -3' miRNA: 3'- -AGuGGUCC------------UGGUCGAACAUCCGU--UGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 29504 | 0.71 | 0.412868 |
Target: 5'- gUUGCCuucuGGGCCAGCUgcUGcgcGGCAACGu -3' miRNA: 3'- -AGUGGu---CCUGGUCGA--ACau-CCGUUGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 25358 | 0.72 | 0.348041 |
Target: 5'- gCugCGcGACCAGCgaaugGUGGGCGGCa -3' miRNA: 3'- aGugGUcCUGGUCGaa---CAUCCGUUGc -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 23187 | 0.72 | 0.338538 |
Target: 5'- gCACCAGGacguucgGCCAGCUcc--GGCAGCGu -3' miRNA: 3'- aGUGGUCC-------UGGUCGAacauCCGUUGC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 13276 | 0.76 | 0.204518 |
Target: 5'- gCACCAGGugCcGCUcgGUAGGCGGuCGa -3' miRNA: 3'- aGUGGUCCugGuCGAa-CAUCCGUU-GC- -5' |
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30429 | 5' | -53.6 | NC_006548.1 | + | 23420 | 0.76 | 0.198892 |
Target: 5'- gCACCAGGACC-GCUUG-GGGCcaGGCGc -3' miRNA: 3'- aGUGGUCCUGGuCGAACaUCCG--UUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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