Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30431 | 5' | -52.6 | NC_006548.1 | + | 20580 | 0.71 | 0.422837 |
Target: 5'- gCGGCGGCAGAGg---CuGCUGCgGCg -3' miRNA: 3'- aGUCGUCGUCUCacuaGuUGACGgUG- -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 38328 | 0.66 | 0.727722 |
Target: 5'- aUCGGCcacccgcucAGaCAGAGcGGUCAGCuUGCCGa -3' miRNA: 3'- -AGUCG---------UC-GUCUCaCUAGUUG-ACGGUg -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 23781 | 0.66 | 0.727722 |
Target: 5'- --cGCAGUuGGGUGAUCG--UGCCAa -3' miRNA: 3'- aguCGUCGuCUCACUAGUugACGGUg -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 33428 | 0.67 | 0.62325 |
Target: 5'- gUCGGCGGCAuGGGUcggaauccaGAUCGcuuuCUGCCGg -3' miRNA: 3'- -AGUCGUCGU-CUCA---------CUAGUu---GACGGUg -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 21428 | 0.68 | 0.576497 |
Target: 5'- cCGGCGGCAGAGUcgcggCAACcGCuCGCc -3' miRNA: 3'- aGUCGUCGUCUCAcua--GUUGaCG-GUG- -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 23171 | 0.69 | 0.553392 |
Target: 5'- cCAGCuccGGCAGcGUGAUguACacgGCCACu -3' miRNA: 3'- aGUCG---UCGUCuCACUAguUGa--CGGUG- -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 11396 | 0.69 | 0.5081 |
Target: 5'- -gAGCGGguGuGuUGAUCAAuCUGCCAa -3' miRNA: 3'- agUCGUCguCuC-ACUAGUU-GACGGUg -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 27161 | 0.69 | 0.497016 |
Target: 5'- aCAGCAGCAuuGAGgcgcUGcgUAcugGCUGCCGCa -3' miRNA: 3'- aGUCGUCGU--CUC----ACuaGU---UGACGGUG- -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 18444 | 0.7 | 0.475186 |
Target: 5'- -uGGCAGCGGcGUGGUCuuCUGCaggGCg -3' miRNA: 3'- agUCGUCGUCuCACUAGuuGACGg--UG- -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 29218 | 0.71 | 0.422837 |
Target: 5'- gUCGGCGGUAGAccGAUgCAggucuagcaguuGCUGCCGCg -3' miRNA: 3'- -AGUCGUCGUCUcaCUA-GU------------UGACGGUG- -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 27306 | 0.71 | 0.393138 |
Target: 5'- uUCAGCAGCAGcc---UUGGCUGCCGCc -3' miRNA: 3'- -AGUCGUCGUCucacuAGUUGACGGUG- -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 13593 | 1.11 | 0.000614 |
Target: 5'- gUCAGCAGCAGAGUGAUCAACUGCCACa -3' miRNA: 3'- -AGUCGUCGUCUCACUAGUUGACGGUG- -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 22543 | 0.7 | 0.442329 |
Target: 5'- aCAGCcgccGGCGGAGgagagccUGAUCGACgGCCAa -3' miRNA: 3'- aGUCG----UCGUCUC-------ACUAGUUGaCGGUg -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 35947 | 0.66 | 0.704917 |
Target: 5'- gUUGGcCGGCAGGGUGGcggcgcgCGACUGCaACg -3' miRNA: 3'- -AGUC-GUCGUCUCACUa------GUUGACGgUG- -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 8821 | 0.66 | 0.693386 |
Target: 5'- -aGGUAGC-GuGUGAUCAGCggaaUGCCAg -3' miRNA: 3'- agUCGUCGuCuCACUAGUUG----ACGGUg -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 16481 | 0.66 | 0.693386 |
Target: 5'- gCuGCGGCAGAGcaccugGAgcagCAGCUGCaGCg -3' miRNA: 3'- aGuCGUCGUCUCa-----CUa---GUUGACGgUG- -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 34325 | 0.66 | 0.681788 |
Target: 5'- aCAGCGGUcagGGAGUGAUugagCAGC-GCgGCa -3' miRNA: 3'- aGUCGUCG---UCUCACUA----GUUGaCGgUG- -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 6500 | 0.66 | 0.681788 |
Target: 5'- -gAGCAGCAGAGguucuUCGACaucGCCGa -3' miRNA: 3'- agUCGUCGUCUCacu--AGUUGa--CGGUg -5' |
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30431 | 5' | -52.6 | NC_006548.1 | + | 1480 | 0.69 | 0.497016 |
Target: 5'- cCGGCAGUGGAGUGGUagcGCU-CCAUg -3' miRNA: 3'- aGUCGUCGUCUCACUAgu-UGAcGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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