Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30432 | 3' | -54.5 | NC_006548.1 | + | 29688 | 0.66 | 0.648658 |
Target: 5'- -aCCAGCaccugcgagcccaUUCGCGGCCGcaaGACUGgcugCGGc -3' miRNA: 3'- aaGGUCG-------------AAGUGCCGGU---UUGGCa---GCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 25626 | 0.66 | 0.646364 |
Target: 5'- gUUCGGCUUCAUGgagcaguaggaucuGCCGuuCCGUCa- -3' miRNA: 3'- aAGGUCGAAGUGC--------------CGGUuuGGCAGcu -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 34134 | 0.66 | 0.603934 |
Target: 5'- -cCCGGCUgUCGgcCGGCCAGuucaccUCGUCGAu -3' miRNA: 3'- aaGGUCGA-AGU--GCCGGUUu-----GGCAGCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 19385 | 0.66 | 0.597073 |
Target: 5'- gUCCAGCUgguagCGCaGCagguugcgcagguGGCCGUCGAg -3' miRNA: 3'- aAGGUCGAa----GUGcCGgu-----------UUGGCAGCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 29492 | 0.67 | 0.581113 |
Target: 5'- -gCCAGCUgcugCGCGGCaa---CGUCGGc -3' miRNA: 3'- aaGGUCGAa---GUGCCGguuugGCAGCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 37344 | 0.67 | 0.569767 |
Target: 5'- -gCCAcGCUgccgacuaGCGGCCAGGCCGagGGu -3' miRNA: 3'- aaGGU-CGAag------UGCCGGUUUGGCagCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 1967 | 0.67 | 0.54725 |
Target: 5'- -cCCcGCUUCACuGGCCAcuUCGUCa- -3' miRNA: 3'- aaGGuCGAAGUG-CCGGUuuGGCAGcu -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 29726 | 0.67 | 0.546131 |
Target: 5'- gUUCGGCUUCGCcgaggguGGCCAGGuCCGUgGu -3' miRNA: 3'- aAGGUCGAAGUG-------CCGGUUU-GGCAgCu -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 22640 | 0.67 | 0.536096 |
Target: 5'- gUCCuggAGUUgaaCGCcGGCCAGuugGCCGUCGAu -3' miRNA: 3'- aAGG---UCGAa--GUG-CCGGUU---UGGCAGCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 36257 | 0.68 | 0.514036 |
Target: 5'- gUCCAGUUcgGCGGCUGGACUG-CGGa -3' miRNA: 3'- aAGGUCGAagUGCCGGUUUGGCaGCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 27744 | 0.69 | 0.450332 |
Target: 5'- --gCAGCUcCA-GGCCAAGCCGuUCGGa -3' miRNA: 3'- aagGUCGAaGUgCCGGUUUGGC-AGCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 14090 | 0.69 | 0.449306 |
Target: 5'- gUCCAGC-UCGCGGCgGAucuccagcgguguGgCGUCGGg -3' miRNA: 3'- aAGGUCGaAGUGCCGgUU-------------UgGCAGCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 24951 | 0.7 | 0.400712 |
Target: 5'- cUUCCAGC-UCACGGgCAGuguGCUGUaCGAc -3' miRNA: 3'- -AAGGUCGaAGUGCCgGUU---UGGCA-GCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 9336 | 0.71 | 0.363613 |
Target: 5'- cUCCAGa-UCGuCGGCUAGGCCGUCc- -3' miRNA: 3'- aAGGUCgaAGU-GCCGGUUUGGCAGcu -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 24665 | 0.71 | 0.354718 |
Target: 5'- gUCCAGCUUCACGaagucGCCGGcuuucagaucGCCcUCGAg -3' miRNA: 3'- aAGGUCGAAGUGC-----CGGUU----------UGGcAGCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 32798 | 0.71 | 0.337392 |
Target: 5'- aUCCAGCUgcuggcaagcgucacCGCGGCCGgcgcacaAACgGUCGAg -3' miRNA: 3'- aAGGUCGAa--------------GUGCCGGU-------UUGgCAGCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 17742 | 0.71 | 0.337392 |
Target: 5'- aUCC-GCUUCuuccaGGCCAAGcauCCGUCGAu -3' miRNA: 3'- aAGGuCGAAGug---CCGGUUU---GGCAGCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 21256 | 0.74 | 0.221082 |
Target: 5'- -aCCGGCagCcuggGCGGCCAGGCCGUcCGAg -3' miRNA: 3'- aaGGUCGaaG----UGCCGGUUUGGCA-GCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 31715 | 0.76 | 0.162452 |
Target: 5'- -cCCGGCgggUCAUcgGGCCAGGCCGUgGAa -3' miRNA: 3'- aaGGUCGa--AGUG--CCGGUUUGGCAgCU- -5' |
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30432 | 3' | -54.5 | NC_006548.1 | + | 28885 | 0.78 | 0.125306 |
Target: 5'- cUUCgGGCcaC-CGGCCAAACCGUCGAg -3' miRNA: 3'- -AAGgUCGaaGuGCCGGUUUGGCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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