Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30433 | 5' | -54.5 | NC_006548.1 | + | 13296 | 0.66 | 0.607485 |
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Target: 5'- gAGGGauagCAGUAGGcgCAGCaccaGGUGCc- -3' miRNA: 3'- -UCCCa---GUCAUCUuaGUCGg---UCGCGuc -5' |
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| 30433 | 5' | -54.5 | NC_006548.1 | + | 33048 | 0.67 | 0.573183 |
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Target: 5'- uGGGGUacaAGcGGGAgccggcCGGCCAGCGCc- -3' miRNA: 3'- -UCCCAg--UCaUCUUa-----GUCGGUCGCGuc -5' |
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| 30433 | 5' | -54.5 | NC_006548.1 | + | 37828 | 0.67 | 0.528224 |
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Target: 5'- cAGGGuUCGGUgAGGccgacgacagAUCAGCU-GCGCAGa -3' miRNA: 3'- -UCCC-AGUCA-UCU----------UAGUCGGuCGCGUC- -5' |
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| 30433 | 5' | -54.5 | NC_006548.1 | + | 18755 | 0.67 | 0.528224 |
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Target: 5'- cAGGGUCGcUAGc-UCGGCCAGUGaCAa -3' miRNA: 3'- -UCCCAGUcAUCuuAGUCGGUCGC-GUc -5' |
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| 30433 | 5' | -54.5 | NC_006548.1 | + | 23837 | 0.68 | 0.51718 |
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Target: 5'- uGGGUgAG-AGAGUCuccugGGCCAGCaGCAc -3' miRNA: 3'- uCCCAgUCaUCUUAG-----UCGGUCG-CGUc -5' |
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| 30433 | 5' | -54.5 | NC_006548.1 | + | 4285 | 0.68 | 0.495379 |
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Target: 5'- cGGGcCAGcuccGGcGUCAGCaCGGUGCAGa -3' miRNA: 3'- uCCCaGUCa---UCuUAGUCG-GUCGCGUC- -5' |
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| 30433 | 5' | -54.5 | NC_006548.1 | + | 18551 | 0.68 | 0.463493 |
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Target: 5'- cGGGUCAuuGgcGAcgcuAUCgAGCCAGCGCu- -3' miRNA: 3'- uCCCAGU--CauCU----UAG-UCGGUCGCGuc -5' |
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| 30433 | 5' | -54.5 | NC_006548.1 | + | 30581 | 0.78 | 0.121799 |
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Target: 5'- -aGGUCAGcacgauuUAcGAGUCGGCCGGCGCGGg -3' miRNA: 3'- ucCCAGUC-------AU-CUUAGUCGGUCGCGUC- -5' |
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| 30433 | 5' | -54.5 | NC_006548.1 | + | 13988 | 1.1 | 0.000563 |
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Target: 5'- cAGGGUCAGUAGAAUCAGCCAGCGCAGg -3' miRNA: 3'- -UCCCAGUCAUCUUAGUCGGUCGCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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