Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30434 | 3' | -51.5 | NC_006548.1 | + | 14254 | 0.66 | 0.833465 |
Target: 5'- uCGAGg-GGCGccuGGCCGaugGCCGGAUGa -3' miRNA: 3'- -GUUUagCCGC---UUGGUgaaCGGCCUAC- -5' |
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30434 | 3' | -51.5 | NC_006548.1 | + | 1420 | 0.66 | 0.814137 |
Target: 5'- aAAGUCGGguacaaGAugCGCgagcUGCCGGAc- -3' miRNA: 3'- gUUUAGCCg-----CUugGUGa---ACGGCCUac -5' |
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30434 | 3' | -51.5 | NC_006548.1 | + | 31140 | 0.66 | 0.793956 |
Target: 5'- ----gCGuaGAGCCACggguUGCCGGAg- -3' miRNA: 3'- guuuaGCcgCUUGGUGa---ACGGCCUac -5' |
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30434 | 3' | -51.5 | NC_006548.1 | + | 23895 | 0.66 | 0.793956 |
Target: 5'- ----aUGGCGAagugcacguuuGCCAUcUGCCGGGUc -3' miRNA: 3'- guuuaGCCGCU-----------UGGUGaACGGCCUAc -5' |
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30434 | 3' | -51.5 | NC_006548.1 | + | 33432 | 0.66 | 0.783577 |
Target: 5'- cCAGGUCGGCGG--CAUggGUCGGAa- -3' miRNA: 3'- -GUUUAGCCGCUugGUGaaCGGCCUac -5' |
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30434 | 3' | -51.5 | NC_006548.1 | + | 29340 | 0.67 | 0.751438 |
Target: 5'- -uGGUCGGCG-GCCA---GCCGGGUa -3' miRNA: 3'- guUUAGCCGCuUGGUgaaCGGCCUAc -5' |
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30434 | 3' | -51.5 | NC_006548.1 | + | 22143 | 0.68 | 0.716951 |
Target: 5'- --cAUCGGCGAagcgcggcugaacAUCGCUcgGCCGGGg- -3' miRNA: 3'- guuUAGCCGCU-------------UGGUGAa-CGGCCUac -5' |
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30434 | 3' | -51.5 | NC_006548.1 | + | 850 | 0.69 | 0.649157 |
Target: 5'- ----cCGGCGGcUCGCUUGCUGGGc- -3' miRNA: 3'- guuuaGCCGCUuGGUGAACGGCCUac -5' |
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30434 | 3' | -51.5 | NC_006548.1 | + | 21536 | 0.72 | 0.457746 |
Target: 5'- ----aCGGCGAucgccaucgccuACCGCaacgGCCGGAUGa -3' miRNA: 3'- guuuaGCCGCU------------UGGUGaa--CGGCCUAC- -5' |
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30434 | 3' | -51.5 | NC_006548.1 | + | 31869 | 0.76 | 0.273235 |
Target: 5'- --cAUCGGCGAAgCGCcugauuucggucugUGCCGGAUGg -3' miRNA: 3'- guuUAGCCGCUUgGUGa-------------ACGGCCUAC- -5' |
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30434 | 3' | -51.5 | NC_006548.1 | + | 4467 | 0.77 | 0.242488 |
Target: 5'- gCAuGUCGGCGAACCGCUcgucacUGCCG-AUGu -3' miRNA: 3'- -GUuUAGCCGCUUGGUGA------ACGGCcUAC- -5' |
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30434 | 3' | -51.5 | NC_006548.1 | + | 14157 | 1.09 | 0.001461 |
Target: 5'- uCAAAUCGGCGAACCACUUGCCGGAUGg -3' miRNA: 3'- -GUUUAGCCGCUUGGUGAACGGCCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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