Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30434 | 5' | -56.8 | NC_006548.1 | + | 14117 | 1.06 | 0.000604 |
Target: 5'- gGUCACCAGGUCACGGUCGGCCAAGUAg -3' miRNA: 3'- -CAGUGGUCCAGUGCCAGCCGGUUCAU- -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 17059 | 0.76 | 0.105398 |
Target: 5'- aUCGCCaAGGUCG-GGcUCGGCCAGGUGg -3' miRNA: 3'- cAGUGG-UCCAGUgCC-AGCCGGUUCAU- -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 20884 | 0.72 | 0.225111 |
Target: 5'- --gGCCAGGUCAcaaagcCGG-CGGCCGAGg- -3' miRNA: 3'- cagUGGUCCAGU------GCCaGCCGGUUCau -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 17197 | 0.68 | 0.403421 |
Target: 5'- uUCACCAGGUCAaGGcgaacaUCGGCCu---- -3' miRNA: 3'- cAGUGGUCCAGUgCC------AGCCGGuucau -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 36808 | 0.67 | 0.432407 |
Target: 5'- -gCGCCAGG-CGCGGcCuuaugcgccuGGCCGGGUGc -3' miRNA: 3'- caGUGGUCCaGUGCCaG----------CCGGUUCAU- -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 9512 | 0.67 | 0.436363 |
Target: 5'- -cCACCAgcaggaggcugcucuGGU-GCGcGUCGGCCAGGUGc -3' miRNA: 3'- caGUGGU---------------CCAgUGC-CAGCCGGUUCAU- -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 25155 | 0.67 | 0.431422 |
Target: 5'- aUCugCAGGUCAuccgccccuggauCGGU-GGCCGGGg- -3' miRNA: 3'- cAGugGUCCAGU-------------GCCAgCCGGUUCau -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 30287 | 0.67 | 0.442336 |
Target: 5'- --gACCAGGUCugGGUucgCGGUgAGGa- -3' miRNA: 3'- cagUGGUCCAGugCCA---GCCGgUUCau -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 24314 | 0.66 | 0.504433 |
Target: 5'- --gGCCGGGUCugGGcCGGCgAGc-- -3' miRNA: 3'- cagUGGUCCAGugCCaGCCGgUUcau -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 18109 | 0.66 | 0.515157 |
Target: 5'- -gCACCuGGcCugGGaCGGCCuGGUGa -3' miRNA: 3'- caGUGGuCCaGugCCaGCCGGuUCAU- -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 16315 | 0.66 | 0.472872 |
Target: 5'- cGUCGCCAcuGGUgGCGaucuccCGGCCAAGg- -3' miRNA: 3'- -CAGUGGU--CCAgUGCca----GCCGGUUCau -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 35495 | 0.66 | 0.504433 |
Target: 5'- uUCACCuGGUCGC---CGGCCAGGc- -3' miRNA: 3'- cAGUGGuCCAGUGccaGCCGGUUCau -5' |
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30434 | 5' | -56.8 | NC_006548.1 | + | 28238 | 0.66 | 0.525971 |
Target: 5'- aUCACgCAGGUCuCGcaCGGCCuGAGUGa -3' miRNA: 3'- cAGUG-GUCCAGuGCcaGCCGG-UUCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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