Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30435 | 3' | -56.2 | NC_006548.1 | + | 1118 | 0.7 | 0.296787 |
Target: 5'- gAGCgagcCUGGCCGUcuGCGcgGCcagcaacgccuguGCGGCg -3' miRNA: 3'- -UCGaa--GACCGGCAu-CGCuaCG-------------UGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 1681 | 0.66 | 0.509167 |
Target: 5'- aAGCUgcauugGGCCGgGGCGGagagaguuaucgGCugGGCa -3' miRNA: 3'- -UCGAaga---CCGGCaUCGCUa-----------CGugCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 7159 | 0.68 | 0.409392 |
Target: 5'- gGGCgugacgaGGCCcUGGUGAUcaGCACGGCg -3' miRNA: 3'- -UCGaaga---CCGGcAUCGCUA--CGUGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 7483 | 0.68 | 0.409392 |
Target: 5'- cAGC--CUGGaaGgcGCGAUGguCGGCa -3' miRNA: 3'- -UCGaaGACCggCauCGCUACguGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 9646 | 0.67 | 0.490006 |
Target: 5'- uGCUcgacaggUGGCUGgguGCGggGCAUGGCg -3' miRNA: 3'- uCGAag-----ACCGGCau-CGCuaCGUGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 11764 | 0.69 | 0.381357 |
Target: 5'- cAGCguccagCUGGUCaUGcGCGgcGCGCGGCg -3' miRNA: 3'- -UCGaa----GACCGGcAU-CGCuaCGUGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 12922 | 0.68 | 0.399905 |
Target: 5'- cAGCacgUUCUGGCuCGcgcugagcGCGGUGCuguGCGGCg -3' miRNA: 3'- -UCG---AAGACCG-GCau------CGCUACG---UGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 14425 | 1.11 | 0.00031 |
Target: 5'- cAGCUUCUGGCCGUAGCGAUGCACGGCu -3' miRNA: 3'- -UCGAAGACCGGCAUCGCUACGUGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 16187 | 0.7 | 0.297548 |
Target: 5'- cGGCUUC-GGC----GCGGUGCGCGGUa -3' miRNA: 3'- -UCGAAGaCCGgcauCGCUACGUGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 16661 | 0.7 | 0.329258 |
Target: 5'- cGCUUCcagGGUgaUGUAGCGGUcGuCGCGGCc -3' miRNA: 3'- uCGAAGa--CCG--GCAUCGCUA-C-GUGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 17468 | 0.8 | 0.068963 |
Target: 5'- cGCagCUGGCCGaAGCGAUGCAgccugucguCGGCa -3' miRNA: 3'- uCGaaGACCGGCaUCGCUACGU---------GCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 18297 | 0.67 | 0.4487 |
Target: 5'- aGGCUcgUCcgGGCCGUccuugcccAGCGAUGC-CaGCu -3' miRNA: 3'- -UCGA--AGa-CCGGCA--------UCGCUACGuGcCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 19045 | 0.66 | 0.511315 |
Target: 5'- cGGC-UCgggGGCCuacaUGGCGccUGCACGGUg -3' miRNA: 3'- -UCGaAGa--CCGGc---AUCGCu-ACGUGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 22081 | 0.66 | 0.543965 |
Target: 5'- cGGCgaa-GGaUgGUGGCGGUGCgACGGCc -3' miRNA: 3'- -UCGaagaCC-GgCAUCGCUACG-UGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 22589 | 0.67 | 0.479508 |
Target: 5'- cGGCggCUGugcGCCgGUAGUGGUugGUGCGGCa -3' miRNA: 3'- -UCGaaGAC---CGG-CAUCGCUA--CGUGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 22875 | 0.69 | 0.349449 |
Target: 5'- aAGCg-CUGGCUGcagcuugcuacgaacUGGCGAUucagucGCGCGGCg -3' miRNA: 3'- -UCGaaGACCGGC---------------AUCGCUA------CGUGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 26978 | 0.71 | 0.282603 |
Target: 5'- gAGCaagCUGGCCGUaAGCuGAgcaGCGuCGGCg -3' miRNA: 3'- -UCGaa-GACCGGCA-UCG-CUa--CGU-GCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 27600 | 0.73 | 0.199684 |
Target: 5'- aGGCca--GGCCGUaaccGGCGAUGC-CGGCg -3' miRNA: 3'- -UCGaagaCCGGCA----UCGCUACGuGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 28018 | 0.66 | 0.522114 |
Target: 5'- gAGUUUCU-GCCGcGGUucgGCGCGGCc -3' miRNA: 3'- -UCGAAGAcCGGCaUCGcuaCGUGCCG- -5' |
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30435 | 3' | -56.2 | NC_006548.1 | + | 33254 | 0.67 | 0.479508 |
Target: 5'- aGGCgggggUUGGCUucc-CGGUGCACGGCu -3' miRNA: 3'- -UCGaa---GACCGGcaucGCUACGUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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