Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30436 | 3' | -56.8 | NC_006548.1 | + | 36514 | 0.66 | 0.495709 |
Target: 5'- gGUUGA-CCuugacCUCGUcgagGUGGGCUCGGCc -3' miRNA: 3'- -UAACUaGGu----GAGCG----CGCCUGAGCCGu -5' |
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30436 | 3' | -56.8 | NC_006548.1 | + | 16484 | 0.67 | 0.454242 |
Target: 5'- ----cUCCACUCGCgGCuGACUaCGGCc -3' miRNA: 3'- uaacuAGGUGAGCG-CGcCUGA-GCCGu -5' |
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30436 | 3' | -56.8 | NC_006548.1 | + | 22612 | 0.67 | 0.453229 |
Target: 5'- --cGuUCCGCUCGCcgaacugccggaaGCGGAaCUgCGGCAg -3' miRNA: 3'- uaaCuAGGUGAGCG-------------CGCCU-GA-GCCGU- -5' |
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30436 | 3' | -56.8 | NC_006548.1 | + | 1244 | 0.67 | 0.434224 |
Target: 5'- --cGGUCUuuGCUUGCGCGcGCggugCGGCAg -3' miRNA: 3'- uaaCUAGG--UGAGCGCGCcUGa---GCCGU- -5' |
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30436 | 3' | -56.8 | NC_006548.1 | + | 22905 | 0.67 | 0.414727 |
Target: 5'- --cGAUUCAgUCGCGCGG---CGGCGa -3' miRNA: 3'- uaaCUAGGUgAGCGCGCCugaGCCGU- -5' |
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30436 | 3' | -56.8 | NC_006548.1 | + | 38260 | 0.68 | 0.386515 |
Target: 5'- --cGAUgCAUUCGCGCuGGucCUCGGUg -3' miRNA: 3'- uaaCUAgGUGAGCGCG-CCu-GAGCCGu -5' |
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30436 | 3' | -56.8 | NC_006548.1 | + | 18025 | 0.68 | 0.368423 |
Target: 5'- --aGAU-CACUCGCGCccgccaGGCUCGGCc -3' miRNA: 3'- uaaCUAgGUGAGCGCGc-----CUGAGCCGu -5' |
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30436 | 3' | -56.8 | NC_006548.1 | + | 12231 | 0.69 | 0.341549 |
Target: 5'- -aUGcgCCGCUCGaaccagcaCGCGGcgauacuGCUCGGCGg -3' miRNA: 3'- uaACuaGGUGAGC--------GCGCC-------UGAGCCGU- -5' |
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30436 | 3' | -56.8 | NC_006548.1 | + | 32200 | 0.7 | 0.286934 |
Target: 5'- gGUUGAcgucaUUCACUCG-GCGuACUCGGCAg -3' miRNA: 3'- -UAACU-----AGGUGAGCgCGCcUGAGCCGU- -5' |
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30436 | 3' | -56.8 | NC_006548.1 | + | 12875 | 0.75 | 0.141333 |
Target: 5'- --cGcgCCGCUCGCGCGGcACU-GGCGa -3' miRNA: 3'- uaaCuaGGUGAGCGCGCC-UGAgCCGU- -5' |
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30436 | 3' | -56.8 | NC_006548.1 | + | 15004 | 1.07 | 0.000592 |
Target: 5'- cAUUGAUCCACUCGCGCGGACUCGGCAg -3' miRNA: 3'- -UAACUAGGUGAGCGCGCCUGAGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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