miRNA: 3'- gagUGGCCGUCGUau--CCGCCAcCUGG- -5'
miRNA: 3'- gAGUGGCCgUCGUA-----------UCCGCCACcUGG- -5'
miRNA: 3'- gagUGGCCGU-CGUa-------UCCGCCACCUGg -5'
miRNA: 3'- -GAGUGGCCGUCGu-------AUCCGcCACC--UGG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30436 | 5' | -58 | NC_006548.1 | + | 8281 | 0.68 | 0.373113 |
|
Target: 5'- --aGCCGGCAGCGacccGGCGGUuGAUUu -3' miRNA: 3'- gagUGGCCGUCGUau--CCGCCAcCUGG- -5' |
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| 30436 | 5' | -58 | NC_006548.1 | + | 5014 | 0.72 | 0.208187 |
|
Target: 5'- aUCACUGG-AGCAUucucgguguucAGGCGGUGcACCa -3' miRNA: 3'- gAGUGGCCgUCGUA-----------UCCGCCACcUGG- -5' |
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| 30436 | 5' | -58 | NC_006548.1 | + | 4613 | 0.73 | 0.172228 |
|
Target: 5'- --gGCUGGCAaGCAguuguccgAGGUGGUGGACg -3' miRNA: 3'- gagUGGCCGU-CGUa-------UCCGCCACCUGg -5' |
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| 30436 | 5' | -58 | NC_006548.1 | + | 845 | 0.73 | 0.163021 |
|
Target: 5'- gCUUGCCGGCGGCucgcuugcUGGGCuGUGGuuGCCa -3' miRNA: 3'- -GAGUGGCCGUCGu-------AUCCGcCACC--UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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