miRNA: 3'- -GAG-UGGCCGUCG----UAUCCGCC--ACC-UGG- -5'
miRNA: 3'- gAGUG-GCCGUCGU---AUCCG-----------CCACCUGG- -5'
miRNA: 3'- gaGUGGCCGUCGuAUCCGCCac-CUGG- -5'
miRNA: 3'- -GAGUGGCCGUCGUAUCCGCCACCUGG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30436 | 5' | -58 | NC_006548.1 | + | 35259 | 0.75 | 0.126866 |
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Target: 5'- cCUCgACUGGCGGCgaugGUGGGCGGccUGGuACCu -3' miRNA: 3'- -GAG-UGGCCGUCG----UAUCCGCC--ACC-UGG- -5' |
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| 30436 | 5' | -58 | NC_006548.1 | + | 22912 | 0.76 | 0.110132 |
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Target: 5'- gUCGCgCGGCGGCGaguUGGGCcggacuaucggGGUGGACUg -3' miRNA: 3'- gAGUG-GCCGUCGU---AUCCG-----------CCACCUGG- -5' |
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| 30436 | 5' | -58 | NC_006548.1 | + | 21258 | 0.78 | 0.07582 |
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Target: 5'- -cUACCGGCAGCcUGGGCGGccaGGCCg -3' miRNA: 3'- gaGUGGCCGUCGuAUCCGCCac-CUGG- -5' |
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| 30436 | 5' | -58 | NC_006548.1 | + | 14970 | 1.12 | 0.000201 |
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Target: 5'- gCUCACCGGCAGCAUAGGCGGUGGACCa -3' miRNA: 3'- -GAGUGGCCGUCGUAUCCGCCACCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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