Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30438 | 3' | -57.1 | NC_006548.1 | + | 8405 | 0.66 | 0.489156 |
Target: 5'- ---cUCGGCCAGGCCGGccucgaugaGGGUcuGCAUg -3' miRNA: 3'- guacAGCUGGUCCGGCC---------UUCGu-CGUA- -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 37411 | 0.66 | 0.478621 |
Target: 5'- ---cUCGGCCuGGCCGcuagucGGCAGCGUg -3' miRNA: 3'- guacAGCUGGuCCGGCcu----UCGUCGUA- -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 9862 | 0.66 | 0.478621 |
Target: 5'- --gGUCGGCCgugaGGGCUuuGGuuGCAGCGg -3' miRNA: 3'- guaCAGCUGG----UCCGG--CCuuCGUCGUa -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 5574 | 0.66 | 0.468199 |
Target: 5'- -----aGGCCAGGCCGGGccGGCuugcGCAa -3' miRNA: 3'- guacagCUGGUCCGGCCU--UCGu---CGUa -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 26545 | 0.66 | 0.457895 |
Target: 5'- uGUGcucUCGAUCauAGcGCCGGAGGCAGUc- -3' miRNA: 3'- gUAC---AGCUGG--UC-CGGCCUUCGUCGua -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 13113 | 0.66 | 0.447714 |
Target: 5'- --gGUCGcgcgcgccaACCAGGCCGaGGAGCAacugcuGCAg -3' miRNA: 3'- guaCAGC---------UGGUCCGGC-CUUCGU------CGUa -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 24026 | 0.66 | 0.437659 |
Target: 5'- --aGUCGcgagcgccuGCCAGGCCGGgcGguGUc- -3' miRNA: 3'- guaCAGC---------UGGUCCGGCCuuCguCGua -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 24773 | 0.67 | 0.417946 |
Target: 5'- ---uUCGAUCAGGCCGGAcGUuGGCGa -3' miRNA: 3'- guacAGCUGGUCCGGCCUuCG-UCGUa -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 22289 | 0.67 | 0.380191 |
Target: 5'- gGUGUCGGCCAGGC--GAucGCGGUAg -3' miRNA: 3'- gUACAGCUGGUCCGgcCUu-CGUCGUa -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 17617 | 0.69 | 0.319828 |
Target: 5'- ----aUGGCCgagGGGCUGGAGGCAGCu- -3' miRNA: 3'- guacaGCUGG---UCCGGCCUUCGUCGua -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 30395 | 0.69 | 0.319828 |
Target: 5'- gGUGcCGcCCAGGCCaGggGCGcGCAg -3' miRNA: 3'- gUACaGCuGGUCCGGcCuuCGU-CGUa -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 26680 | 0.69 | 0.311022 |
Target: 5'- --aGUCGACCGacaucaccuacacGGUCGGAGGUGGCc- -3' miRNA: 3'- guaCAGCUGGU-------------CCGGCCUUCGUCGua -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 33446 | 0.71 | 0.233659 |
Target: 5'- uGUGUCGACCAGcaCCaGGucGGCGGCAUg -3' miRNA: 3'- gUACAGCUGGUCc-GG-CCu-UCGUCGUA- -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 4621 | 0.73 | 0.172647 |
Target: 5'- ---uUCGGCCGGGCUGGcAAGCAGUu- -3' miRNA: 3'- guacAGCUGGUCCGGCC-UUCGUCGua -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 16472 | 0.73 | 0.172164 |
Target: 5'- ---aUCGACCAGGCUGcggcagagcaccuGGAGCAGCAg -3' miRNA: 3'- guacAGCUGGUCCGGC-------------CUUCGUCGUa -5' |
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30438 | 3' | -57.1 | NC_006548.1 | + | 16145 | 1.06 | 0.000573 |
Target: 5'- gCAUGUCGACCAGGCCGGAAGCAGCAUg -3' miRNA: 3'- -GUACAGCUGGUCCGGCCUUCGUCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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