Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30438 | 5' | -58.5 | NC_006548.1 | + | 20469 | 0.7 | 0.260974 |
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Target: 5'- cGGugCCAGUGCCCAGg---CGUCGu- -3' miRNA: 3'- -UCugGGUCGCGGGUCuugaGCAGCcg -5' |
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| 30438 | 5' | -58.5 | NC_006548.1 | + | 9344 | 0.7 | 0.247851 |
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Target: 5'- ---gCCAGCGUcuCCAGA--UCGUCGGCu -3' miRNA: 3'- ucugGGUCGCG--GGUCUugAGCAGCCG- -5' |
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| 30438 | 5' | -58.5 | NC_006548.1 | + | 28010 | 0.7 | 0.241497 |
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Target: 5'- uGugUUGGUGCCCAGGGcCUUGgCGGCa -3' miRNA: 3'- uCugGGUCGCGGGUCUU-GAGCaGCCG- -5' |
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| 30438 | 5' | -58.5 | NC_006548.1 | + | 12630 | 0.71 | 0.229196 |
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Target: 5'- cGGCCgAGCGCCCGGcguugaagcGCUCGaCGGa -3' miRNA: 3'- uCUGGgUCGCGGGUCu--------UGAGCaGCCg -5' |
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| 30438 | 5' | -58.5 | NC_006548.1 | + | 16823 | 0.71 | 0.223247 |
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Target: 5'- cAGGCCCAGU-CCaUAGGGCUCaucgucguuGUCGGCg -3' miRNA: 3'- -UCUGGGUCGcGG-GUCUUGAG---------CAGCCG- -5' |
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| 30438 | 5' | -58.5 | NC_006548.1 | + | 7349 | 0.73 | 0.157253 |
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Target: 5'- -cACCCAGCGCCUGGuugacCUCGacCGGCa -3' miRNA: 3'- ucUGGGUCGCGGGUCuu---GAGCa-GCCG- -5' |
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| 30438 | 5' | -58.5 | NC_006548.1 | + | 19980 | 0.74 | 0.13694 |
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Target: 5'- cGAUCCGGCGCUCAGcgaggccccGCUCGucaUCGGCc -3' miRNA: 3'- uCUGGGUCGCGGGUCu--------UGAGC---AGCCG- -5' |
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| 30438 | 5' | -58.5 | NC_006548.1 | + | 22859 | 0.8 | 0.051752 |
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Target: 5'- cAGcACCUgguAGCGCCCGGc-CUCGUCGGCg -3' miRNA: 3'- -UC-UGGG---UCGCGGGUCuuGAGCAGCCG- -5' |
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| 30438 | 5' | -58.5 | NC_006548.1 | + | 16111 | 1.12 | 0.000195 |
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Target: 5'- cAGACCCAGCGCCCAGAACUCGUCGGCa -3' miRNA: 3'- -UCUGGGUCGCGGGUCUUGAGCAGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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