Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30439 | 3' | -58.1 | NC_006548.1 | + | 13267 | 0.66 | 0.500548 |
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Target: 5'- gCCGCucGGUaggcGGUCGAUacaUGGGCGAUUc -3' miRNA: 3'- -GGCG--UCGga--CCAGCUA---GCCCGCUAGu -5' |
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| 30439 | 3' | -58.1 | NC_006548.1 | + | 37318 | 0.66 | 0.49023 |
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Target: 5'- -gGCAGCC-GGUCGucaagGGGCGcGUCGc -3' miRNA: 3'- ggCGUCGGaCCAGCuag--CCCGC-UAGU- -5' |
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| 30439 | 3' | -58.1 | NC_006548.1 | + | 6082 | 0.66 | 0.469898 |
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Target: 5'- uUCGC-GCCaggGGUUG--CGGGUGAUCAg -3' miRNA: 3'- -GGCGuCGGa--CCAGCuaGCCCGCUAGU- -5' |
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| 30439 | 3' | -58.1 | NC_006548.1 | + | 17496 | 0.66 | 0.450005 |
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Target: 5'- gCCGCuGCUgGGUCGA-CGGcUGAUCGc -3' miRNA: 3'- -GGCGuCGGaCCAGCUaGCCcGCUAGU- -5' |
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| 30439 | 3' | -58.1 | NC_006548.1 | + | 36280 | 0.66 | 0.450005 |
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Target: 5'- cCCGCGG---GGUgCGGUCGGGCGGg-- -3' miRNA: 3'- -GGCGUCggaCCA-GCUAGCCCGCUagu -5' |
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| 30439 | 3' | -58.1 | NC_006548.1 | + | 1452 | 0.67 | 0.440234 |
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Target: 5'- -aGCAGuuCCUGGcCGGcggCGGGCaGAUCGa -3' miRNA: 3'- ggCGUC--GGACCaGCUa--GCCCG-CUAGU- -5' |
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| 30439 | 3' | -58.1 | NC_006548.1 | + | 13799 | 0.67 | 0.42106 |
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Target: 5'- uCCGCGGCCaGaGcgUGAUCGaGGCGAagcUCGa -3' miRNA: 3'- -GGCGUCGGaC-Ca-GCUAGC-CCGCU---AGU- -5' |
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| 30439 | 3' | -58.1 | NC_006548.1 | + | 15212 | 0.69 | 0.341383 |
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Target: 5'- cCCGCaAGCUaUGGUCGAUugccuaucCGGGC-AUCAc -3' miRNA: 3'- -GGCG-UCGG-ACCAGCUA--------GCCCGcUAGU- -5' |
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| 30439 | 3' | -58.1 | NC_006548.1 | + | 16525 | 1.09 | 0.000339 |
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Target: 5'- gCCGCAGCCUGGUCGAUCGGGCGAUCAc -3' miRNA: 3'- -GGCGUCGGACCAGCUAGCCCGCUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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