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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30440 | 3' | -50.1 | NC_006548.1 | + | 28608 | 0.67 | 0.869205 |
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Target: 5'- -gCGGGUagcGCCGCGaccUGGCCGgcgccGAGCAg -3' miRNA: 3'- gaGCCUA---UGGCGC---AUUGGCau---CUUGU- -5' |
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| 30440 | 3' | -50.1 | NC_006548.1 | + | 6554 | 0.68 | 0.793956 |
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Target: 5'- aUCaGGAUG-CGUGgcacGCCGUGGAGCAu -3' miRNA: 3'- gAG-CCUAUgGCGCau--UGGCAUCUUGU- -5' |
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| 30440 | 3' | -50.1 | NC_006548.1 | + | 29164 | 0.69 | 0.762304 |
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Target: 5'- aUCGGucUACCGCc-GACCagGUAGAGCAa -3' miRNA: 3'- gAGCCu-AUGGCGcaUUGG--CAUCUUGU- -5' |
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| 30440 | 3' | -50.1 | NC_006548.1 | + | 17247 | 0.71 | 0.660761 |
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Target: 5'- -aCGGGUguAUCGCGUGAUCGaAGAGCc -3' miRNA: 3'- gaGCCUA--UGGCGCAUUGGCaUCUUGu -5' |
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| 30440 | 3' | -50.1 | NC_006548.1 | + | 33535 | 0.74 | 0.48977 |
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Target: 5'- -gCGGAUAUCGCGccGGCCG-GGAGCAu -3' miRNA: 3'- gaGCCUAUGGCGCa-UUGGCaUCUUGU- -5' |
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| 30440 | 3' | -50.1 | NC_006548.1 | + | 32560 | 0.76 | 0.378292 |
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Target: 5'- -cCGGAUcACCGCGUGGCCGUuu-GCGc -3' miRNA: 3'- gaGCCUA-UGGCGCAUUGGCAucuUGU- -5' |
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| 30440 | 3' | -50.1 | NC_006548.1 | + | 16620 | 1.09 | 0.002421 |
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Target: 5'- gCUCGGAUACCGCGUAACCGUAGAACAc -3' miRNA: 3'- -GAGCCUAUGGCGCAUUGGCAUCUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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