Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30441 | 5' | -50.9 | NC_006548.1 | + | 14077 | 0.66 | 0.829012 |
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Target: 5'- cGGAUCuccagCgGUGUGGCGU-CGGGGUa -3' miRNA: 3'- -CUUAGuca--GaCAUACCGUAcGUCCCG- -5' |
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| 30441 | 5' | -50.9 | NC_006548.1 | + | 18454 | 0.67 | 0.730188 |
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Target: 5'- ---cCGGUUcGgGUGGCAgcggcguggucuucUGCAGGGCg -3' miRNA: 3'- cuuaGUCAGaCaUACCGU--------------ACGUCCCG- -5' |
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| 30441 | 5' | -50.9 | NC_006548.1 | + | 2464 | 0.68 | 0.710733 |
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Target: 5'- -cAUCAGUUUGUG-GGCAUgacGCAGcGCg -3' miRNA: 3'- cuUAGUCAGACAUaCCGUA---CGUCcCG- -5' |
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| 30441 | 5' | -50.9 | NC_006548.1 | + | 32585 | 0.68 | 0.710733 |
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Target: 5'- ---cCGGaCUGggcugAUGGCGUGCAGGaGUc -3' miRNA: 3'- cuuaGUCaGACa----UACCGUACGUCC-CG- -5' |
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| 30441 | 5' | -50.9 | NC_006548.1 | + | 9631 | 0.7 | 0.589743 |
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Target: 5'- gGGAUCAgGUCgauaugcucgacagGUGGCuggGUGCGGGGCa -3' miRNA: 3'- -CUUAGU-CAGaca-----------UACCG---UACGUCCCG- -5' |
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| 30441 | 5' | -50.9 | NC_006548.1 | + | 16997 | 1.12 | 0.000929 |
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Target: 5'- cGAAUCAGUCUGUAUGGCAUGCAGGGCc -3' miRNA: 3'- -CUUAGUCAGACAUACCGUACGUCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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