Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30442 | 3' | -54.9 | NC_006548.1 | + | 9517 | 0.67 | 0.55803 |
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Target: 5'- --aGCAGGaggcugcucuGGUgCGCGUCGGCCa -3' miRNA: 3'- gagCGUCUaau-------CUAgGCGCAGCCGGa -5' |
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| 30442 | 3' | -54.9 | NC_006548.1 | + | 32924 | 0.67 | 0.538137 |
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Target: 5'- gUCG-AGAUUcAGAUcugaCCGCGUCGGCg- -3' miRNA: 3'- gAGCgUCUAA-UCUA----GGCGCAGCCGga -5' |
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| 30442 | 3' | -54.9 | NC_006548.1 | + | 28611 | 0.7 | 0.404076 |
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Target: 5'- gUCGCGGG-UAGcgCCGCGaccUGGCCg -3' miRNA: 3'- gAGCGUCUaAUCuaGGCGCa--GCCGGa -5' |
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| 30442 | 3' | -54.9 | NC_006548.1 | + | 37756 | 0.72 | 0.285629 |
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Target: 5'- -gCGCAGcu--GAUCUGuCGUCGGCCUc -3' miRNA: 3'- gaGCGUCuaauCUAGGC-GCAGCCGGA- -5' |
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| 30442 | 3' | -54.9 | NC_006548.1 | + | 36717 | 0.73 | 0.278305 |
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Target: 5'- cCUCGUAGAaccAGAUCagCGCGcCGGCCUc -3' miRNA: 3'- -GAGCGUCUaa-UCUAG--GCGCaGCCGGA- -5' |
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| 30442 | 3' | -54.9 | NC_006548.1 | + | 17223 | 1.08 | 0.000855 |
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Target: 5'- aCUCGCAGAUUAGAUCCGCGUCGGCCUu -3' miRNA: 3'- -GAGCGUCUAAUCUAGGCGCAGCCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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