Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30442 | 5' | -50.8 | NC_006548.1 | + | 36530 | 0.77 | 0.257626 |
Target: 5'- gUCGAGGUGGGC-UCGGCC-GCGauGAUCg -3' miRNA: 3'- -AGUUCCGCUUGuAGCCGGaCGU--UUAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 34067 | 0.77 | 0.250617 |
Target: 5'- aCGAGGUGAACuggcCGGCCgacagccgggUGCAGAUCa -3' miRNA: 3'- aGUUCCGCUUGua--GCCGG----------ACGUUUAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 15814 | 0.66 | 0.807905 |
Target: 5'- aUCGgcuGGGCGGACGacgcucaggaccaguUCGGCgaGCAccguGUCg -3' miRNA: 3'- -AGU---UCCGCUUGU---------------AGCCGgaCGUu---UAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 20437 | 0.66 | 0.803825 |
Target: 5'- aUCGAGGaGGACGcgCGGCgaGCAG-UCa -3' miRNA: 3'- -AGUUCCgCUUGUa-GCCGgaCGUUuAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 24524 | 0.67 | 0.793483 |
Target: 5'- aUCGcuguGGCGAuc-UgGGCCUcGCAGAUCg -3' miRNA: 3'- -AGUu---CCGCUuguAgCCGGA-CGUUUAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 22153 | 0.67 | 0.793483 |
Target: 5'- gCGcGGCuGAACAucgcUCGGCCgggGCAGAa- -3' miRNA: 3'- aGUuCCG-CUUGU----AGCCGGa--CGUUUag -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 36354 | 0.68 | 0.72785 |
Target: 5'- gCAu-GCGGcCGUcCGGCCUGCAGAUg -3' miRNA: 3'- aGUucCGCUuGUA-GCCGGACGUUUAg -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 15328 | 0.68 | 0.704945 |
Target: 5'- -gAGGGCGGcaauccgaagaAUAUCaGCCUGCAcacGGUCa -3' miRNA: 3'- agUUCCGCU-----------UGUAGcCGGACGU---UUAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 22313 | 0.69 | 0.681713 |
Target: 5'- cCGAucCGAACAUCGGUCUGCGc--- -3' miRNA: 3'- aGUUccGCUUGUAGCCGGACGUuuag -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 7366 | 0.7 | 0.572461 |
Target: 5'- cCAGGGCGGccAUAUCGGCCcccacccagucgggUGCcuGGUCg -3' miRNA: 3'- aGUUCCGCU--UGUAGCCGG--------------ACGu-UUAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 28068 | 0.71 | 0.564324 |
Target: 5'- cUCAcagcAGGCG-GCuggCGGCCUGCAGGc- -3' miRNA: 3'- -AGU----UCCGCuUGua-GCCGGACGUUUag -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 17063 | 0.71 | 0.518514 |
Target: 5'- cCAAGGuCGGGC-UCGGCCaggugGCGAGUa -3' miRNA: 3'- aGUUCC-GCUUGuAGCCGGa----CGUUUAg -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 9011 | 0.73 | 0.411657 |
Target: 5'- aCAGGGCGAGCG-CGGCaccCAGGUCu -3' miRNA: 3'- aGUUCCGCUUGUaGCCGgacGUUUAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 34996 | 0.74 | 0.36358 |
Target: 5'- -gGAGGCGAGCAaugggCGGCCguaGCAAAg- -3' miRNA: 3'- agUUCCGCUUGUa----GCCGGa--CGUUUag -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 19283 | 0.66 | 0.843007 |
Target: 5'- cCAAGGCGugcCGUCaggcgauaccugGGCCUG-GAAUCg -3' miRNA: 3'- aGUUCCGCuu-GUAG------------CCGGACgUUUAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 29009 | 0.66 | 0.833564 |
Target: 5'- cCGuGGCGAGCAgacGCuuCUGCAGGUCg -3' miRNA: 3'- aGUuCCGCUUGUagcCG--GACGUUUAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 22297 | 0.66 | 0.813962 |
Target: 5'- cCGuuGCGggUGUCGGCCagGCG-AUCg -3' miRNA: 3'- aGUucCGCuuGUAGCCGGa-CGUuUAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 29482 | 0.67 | 0.76135 |
Target: 5'- gCGcGGC-AACGUCGGCCaGCG-AUCg -3' miRNA: 3'- aGUuCCGcUUGUAGCCGGaCGUuUAG- -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 6845 | 0.67 | 0.76135 |
Target: 5'- -uGGGGCGAACAagaaggcuagCGGCCUaCAGGUg -3' miRNA: 3'- agUUCCGCUUGUa---------GCCGGAcGUUUAg -5' |
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30442 | 5' | -50.8 | NC_006548.1 | + | 16656 | 0.69 | 0.681713 |
Target: 5'- cCAGGGUGAuguaGCGgucgucgCGGCC-GUAGAUCa -3' miRNA: 3'- aGUUCCGCU----UGUa------GCCGGaCGUUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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