Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30443 | 3' | -54.2 | NC_006548.1 | + | 22336 | 0.66 | 0.678592 |
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Target: 5'- gGugGcCAgcGCGUCCGuGUC-GGUGAGu -3' miRNA: 3'- -CugCaGUaaUGCAGGC-CGGuCCACUC- -5' |
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| 30443 | 3' | -54.2 | NC_006548.1 | + | 32465 | 0.66 | 0.655998 |
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Target: 5'- cGGCGgcg--ACGgcugCCGGCCAGGcUGAu -3' miRNA: 3'- -CUGCaguaaUGCa---GGCCGGUCC-ACUc -5' |
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| 30443 | 3' | -54.2 | NC_006548.1 | + | 21278 | 0.67 | 0.621957 |
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Target: 5'- cGACGUCAUc-CGuUCCGGCCuggcgccGGUcGAGu -3' miRNA: 3'- -CUGCAGUAauGC-AGGCCGGu------CCA-CUC- -5' |
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| 30443 | 3' | -54.2 | NC_006548.1 | + | 32880 | 0.67 | 0.621957 |
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Target: 5'- cGAcCGUUugUGCG-CCGGCCGcGGUGAc -3' miRNA: 3'- -CU-GCAGuaAUGCaGGCCGGU-CCACUc -5' |
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| 30443 | 3' | -54.2 | NC_006548.1 | + | 22304 | 0.67 | 0.588 |
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Target: 5'- gGugGUgcCGUUGCGggugUCGGCCAGGcGAu -3' miRNA: 3'- -CugCA--GUAAUGCa---GGCCGGUCCaCUc -5' |
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| 30443 | 3' | -54.2 | NC_006548.1 | + | 13428 | 0.68 | 0.565542 |
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Target: 5'- cGGCGUCAgaaccgGCGgCgGGCCAGGUc-- -3' miRNA: 3'- -CUGCAGUaa----UGCaGgCCGGUCCAcuc -5' |
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| 30443 | 3' | -54.2 | NC_006548.1 | + | 35222 | 0.68 | 0.532306 |
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Target: 5'- gGACGUCAUguucgGCGccCCGGaCCAaccGGUGAa -3' miRNA: 3'- -CUGCAGUAa----UGCa-GGCC-GGU---CCACUc -5' |
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| 30443 | 3' | -54.2 | NC_006548.1 | + | 26336 | 0.69 | 0.468199 |
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Target: 5'- gGAUGUCGgucaGCGuuUCCGGCCGGGUc-- -3' miRNA: 3'- -CUGCAGUaa--UGC--AGGCCGGUCCAcuc -5' |
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| 30443 | 3' | -54.2 | NC_006548.1 | + | 17702 | 1.08 | 0.001061 |
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Target: 5'- gGACGUCAUUACGUCCGGCCAGGUGAGc -3' miRNA: 3'- -CUGCAGUAAUGCAGGCCGGUCCACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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