Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30443 | 5' | -58.2 | NC_006548.1 | + | 17262 | 0.66 | 0.469943 |
Target: 5'- uCCaCUCGGUCAgccaacgGGCC-GGGCcgagGUUGa -3' miRNA: 3'- -GG-GAGCCGGUa------CCGGaCCCGca--UAAC- -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 12277 | 0.66 | 0.449898 |
Target: 5'- gCCCgUCGGCgCAcagGGCCUGGcGCa----- -3' miRNA: 3'- -GGG-AGCCG-GUa--CCGGACC-CGcauaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 8469 | 0.67 | 0.411276 |
Target: 5'- aCCCUCaucgaGGCC--GGCCUGGccgaGCGUAg-- -3' miRNA: 3'- -GGGAG-----CCGGuaCCGGACC----CGCAUaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 23550 | 0.67 | 0.409399 |
Target: 5'- gCCCUCGGCgAUGaGCUgcaggaauuucaGGGCGUc--- -3' miRNA: 3'- -GGGAGCCGgUAC-CGGa-----------CCCGCAuaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 17440 | 0.68 | 0.366002 |
Target: 5'- gCCgUGGCCucGGCCUGGGUGg---- -3' miRNA: 3'- gGGaGCCGGuaCCGGACCCGCauaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 5488 | 0.68 | 0.340536 |
Target: 5'- aCCgCUCGaGCCAggucGGCCUGGGUc----- -3' miRNA: 3'- -GG-GAGC-CGGUa---CCGGACCCGcauaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 667 | 0.68 | 0.340536 |
Target: 5'- -gCUCuGCCAgGGCCUGGGCa----- -3' miRNA: 3'- ggGAGcCGGUaCCGGACCCGcauaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 14694 | 0.68 | 0.332337 |
Target: 5'- uUCCUCGGCCucuGCCUGGaacuucuucaGCGUGa-- -3' miRNA: 3'- -GGGAGCCGGuacCGGACC----------CGCAUaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 30328 | 0.7 | 0.265106 |
Target: 5'- aCUCUgCGcGCCccUGGCCUGGGCGg---- -3' miRNA: 3'- -GGGA-GC-CGGu-ACCGGACCCGCauaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 5624 | 0.7 | 0.238951 |
Target: 5'- gCC-CGGCC-UGGCCUGcGCGUAg-- -3' miRNA: 3'- gGGaGCCGGuACCGGACcCGCAUaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 13064 | 0.71 | 0.220779 |
Target: 5'- aCCCUCGGCgc-GGCCgGuGGCGUAc-- -3' miRNA: 3'- -GGGAGCCGguaCCGGaC-CCGCAUaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 37414 | 0.72 | 0.198394 |
Target: 5'- aCCCUCGGCC-UGGCCgcuagucGGcaGCGUGg-- -3' miRNA: 3'- -GGGAGCCGGuACCGGa------CC--CGCAUaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 17608 | 0.72 | 0.178014 |
Target: 5'- gCCCa-GGCCAUGGCCgagGGGCu----- -3' miRNA: 3'- -GGGagCCGGUACCGGa--CCCGcauaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 19552 | 0.79 | 0.058743 |
Target: 5'- -gCUCGGCCAUGGCUUGGGaCGUc--- -3' miRNA: 3'- ggGAGCCGGUACCGGACCC-GCAuaac -5' |
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30443 | 5' | -58.2 | NC_006548.1 | + | 17665 | 1.1 | 0.000259 |
Target: 5'- cCCCUCGGCCAUGGCCUGGGCGUAUUGa -3' miRNA: 3'- -GGGAGCCGGUACCGGACCCGCAUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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