Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30444 | 3' | -53.8 | NC_006548.1 | + | 22326 | 0.66 | 0.704726 |
Target: 5'- cCCAcUGGGCGGucaggccgaagGUGGUGcCGUugcgggugUCgGCCAg -3' miRNA: 3'- -GGUaACCCGCC-----------UAUCAC-GCA--------AG-CGGU- -5' |
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30444 | 3' | -53.8 | NC_006548.1 | + | 29562 | 0.67 | 0.65966 |
Target: 5'- aCCGca-GGCGGAacucugguggcUGGUGCGgcUGCCAu -3' miRNA: 3'- -GGUaacCCGCCU-----------AUCACGCaaGCGGU- -5' |
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30444 | 3' | -53.8 | NC_006548.1 | + | 24387 | 0.67 | 0.65966 |
Target: 5'- gCCAUUGGGC----AG-GCGcUCGCCGg -3' miRNA: 3'- -GGUAACCCGccuaUCaCGCaAGCGGU- -5' |
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30444 | 3' | -53.8 | NC_006548.1 | + | 31769 | 0.67 | 0.636916 |
Target: 5'- aCGUUGcGCuGGUAGaUGCGUcCGCCAa -3' miRNA: 3'- gGUAACcCGcCUAUC-ACGCAaGCGGU- -5' |
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30444 | 3' | -53.8 | NC_006548.1 | + | 6960 | 0.74 | 0.254558 |
Target: 5'- aCAUcGGGCGGAUcucuccGGUGCGU-CGUCGa -3' miRNA: 3'- gGUAaCCCGCCUA------UCACGCAaGCGGU- -5' |
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30444 | 3' | -53.8 | NC_006548.1 | + | 18219 | 1.1 | 0.000748 |
Target: 5'- gCCAUUGGGCGGAUAGUGCGUUCGCCAc -3' miRNA: 3'- -GGUAACCCGCCUAUCACGCAAGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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