Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30448 | 5' | -60.1 | NC_006548.1 | + | 34848 | 0.66 | 0.393777 |
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Target: 5'- cGGCCGggUUACUGCGUAuccGGuGCGCgcgCCu -3' miRNA: 3'- -UCGGC--AGUGGCGCAU---CC-CGCGaa-GGc -5' |
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| 30448 | 5' | -60.1 | NC_006548.1 | + | 8781 | 0.66 | 0.393777 |
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Target: 5'- cAGaCCGUCcugcaugacGCCGU-UGGGGCGCUcCUGg -3' miRNA: 3'- -UC-GGCAG---------UGGCGcAUCCCGCGAaGGC- -5' |
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| 30448 | 5' | -60.1 | NC_006548.1 | + | 1127 | 0.66 | 0.373427 |
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Target: 5'- uGGCCGUCugCGCGgccagcaacgccuguGcGGCGaucaUCCGg -3' miRNA: 3'- -UCGGCAGugGCGCau-------------C-CCGCga--AGGC- -5' |
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| 30448 | 5' | -60.1 | NC_006548.1 | + | 14498 | 0.66 | 0.367377 |
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Target: 5'- aGGUCGUCGCCGCcu-GGGCacggaucaGCUUggCCGc -3' miRNA: 3'- -UCGGCAGUGGCGcauCCCG--------CGAA--GGC- -5' |
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| 30448 | 5' | -60.1 | NC_006548.1 | + | 12802 | 0.66 | 0.358851 |
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Target: 5'- gAGCUGUauugcucgccagUGCCGCGcgAGcGGCGCgguaagUCCGg -3' miRNA: 3'- -UCGGCA------------GUGGCGCa-UC-CCGCGa-----AGGC- -5' |
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| 30448 | 5' | -60.1 | NC_006548.1 | + | 23984 | 0.66 | 0.358851 |
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Target: 5'- gGGCCuugcguacGUCACCGaGUacGGGGCGCaggucaUCCGc -3' miRNA: 3'- -UCGG--------CAGUGGCgCA--UCCCGCGa-----AGGC- -5' |
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| 30448 | 5' | -60.1 | NC_006548.1 | + | 18264 | 0.67 | 0.310648 |
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Target: 5'- cAGCuCGUCcuCCGCGUA-GGCGCUgaCUGa -3' miRNA: 3'- -UCG-GCAGu-GGCGCAUcCCGCGAa-GGC- -5' |
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| 30448 | 5' | -60.1 | NC_006548.1 | + | 32414 | 0.69 | 0.234797 |
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Target: 5'- cAGCCGUCGCCGCcguugccGUucgcggcgcugcAGGaGCGCaUCUGg -3' miRNA: 3'- -UCGGCAGUGGCG-------CA------------UCC-CGCGaAGGC- -5' |
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| 30448 | 5' | -60.1 | NC_006548.1 | + | 20379 | 1.07 | 0.000283 |
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Target: 5'- gAGCCGUCACCGCGUAGGGCGCUUCCGc -3' miRNA: 3'- -UCGGCAGUGGCGCAUCCCGCGAAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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