Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30455 | 5' | -54.8 | NC_006548.1 | + | 37984 | 0.66 | 0.646811 |
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Target: 5'- cCGCUUCGGGAuugcgcgcCUGGCCuaagCGUUu- -3' miRNA: 3'- -GCGAAGCCCU--------GACCGGugaaGUAGcg -5' |
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| 30455 | 5' | -54.8 | NC_006548.1 | + | 2967 | 0.66 | 0.646811 |
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Target: 5'- uCGCUacaaggucauUCGGGcCaUGGCCGacaaCAUCGCc -3' miRNA: 3'- -GCGA----------AGCCCuG-ACCGGUgaa-GUAGCG- -5' |
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| 30455 | 5' | -54.8 | NC_006548.1 | + | 29994 | 0.68 | 0.524042 |
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Target: 5'- uGCgggugCGGGaauGCUGGCUAUgccgccCGUCGCg -3' miRNA: 3'- gCGaa---GCCC---UGACCGGUGaa----GUAGCG- -5' |
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| 30455 | 5' | -54.8 | NC_006548.1 | + | 1969 | 0.75 | 0.19007 |
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Target: 5'- cCGCUUC---ACUGGCCACUUCGUCa- -3' miRNA: 3'- -GCGAAGcccUGACCGGUGAAGUAGcg -5' |
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| 30455 | 5' | -54.8 | NC_006548.1 | + | 29962 | 0.77 | 0.152015 |
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Target: 5'- uGCUcCGGGcCUGGCUGCUUCG-CGCc -3' miRNA: 3'- gCGAaGCCCuGACCGGUGAAGUaGCG- -5' |
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| 30455 | 5' | -54.8 | NC_006548.1 | + | 22809 | 1.11 | 0.000513 |
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Target: 5'- cCGCUUCGGGACUGGCCACUUCAUCGCc -3' miRNA: 3'- -GCGAAGCCCUGACCGGUGAAGUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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