Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30456 | 3' | -58.4 | NC_006548.1 | + | 28955 | 0.66 | 0.440053 |
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Target: 5'- cGG-CCGCCUCGAcGGUuuGGCCgGu- -3' miRNA: 3'- -UCaGGUGGGGCUaUCAggCCGGgUug -5' |
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| 30456 | 3' | -58.4 | NC_006548.1 | + | 20991 | 0.67 | 0.401946 |
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Target: 5'- aAGUCCACCUguuUGGgcAGUUCGG-CCAGCa -3' miRNA: 3'- -UCAGGUGGG---GCUa-UCAGGCCgGGUUG- -5' |
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| 30456 | 3' | -58.4 | NC_006548.1 | + | 35286 | 0.67 | 0.392752 |
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Target: 5'- uGGUCCgggGCgCCGAacaugacGUCCagcGGCCCAACg -3' miRNA: 3'- -UCAGG---UGgGGCUau-----CAGG---CCGGGUUG- -5' |
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| 30456 | 3' | -58.4 | NC_006548.1 | + | 15736 | 0.68 | 0.340536 |
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Target: 5'- aAGUCCGCCCaggaagCGAUccUCUGGCCCu-- -3' miRNA: 3'- -UCAGGUGGG------GCUAucAGGCCGGGuug -5' |
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| 30456 | 3' | -58.4 | NC_006548.1 | + | 17377 | 0.68 | 0.332337 |
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Target: 5'- gAGUCCACCCaGGccgAGgccaCGGCgCCGACg -3' miRNA: 3'- -UCAGGUGGGgCUa--UCag--GCCG-GGUUG- -5' |
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| 30456 | 3' | -58.4 | NC_006548.1 | + | 6260 | 0.68 | 0.316377 |
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Target: 5'- uGGUCCGCCCgGAUGGUCa--UCCAGa -3' miRNA: 3'- -UCAGGUGGGgCUAUCAGgccGGGUUg -5' |
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| 30456 | 3' | -58.4 | NC_006548.1 | + | 5686 | 0.69 | 0.279032 |
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Target: 5'- --cCCACCCCGAaGGcgugCCGGaagaCCAGCu -3' miRNA: 3'- ucaGGUGGGGCUaUCa---GGCCg---GGUUG- -5' |
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| 30456 | 3' | -58.4 | NC_006548.1 | + | 8924 | 0.72 | 0.173218 |
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Target: 5'- uGGUCUGCCCCaGGUGGUCgaUGGCCaGGCg -3' miRNA: 3'- -UCAGGUGGGG-CUAUCAG--GCCGGgUUG- -5' |
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| 30456 | 3' | -58.4 | NC_006548.1 | + | 22990 | 1.09 | 0.000311 |
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Target: 5'- cAGUCCACCCCGAUAGUCCGGCCCAACu -3' miRNA: 3'- -UCAGGUGGGGCUAUCAGGCCGGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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