Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30457 | 5' | -56.4 | NC_006548.1 | + | 32765 | 0.66 | 0.520639 |
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Target: 5'- gGGCGGaUCUGGGcGCUG-CCGGuGUGGc -3' miRNA: 3'- -UCGUC-GGACCCaCGAUaGGUC-CAUCu -5' |
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| 30457 | 5' | -56.4 | NC_006548.1 | + | 36816 | 0.66 | 0.519547 |
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Target: 5'- cGCGGCCUuaugcgccuggccGGGUGCUGgugUguGGUGu- -3' miRNA: 3'- uCGUCGGA-------------CCCACGAUa--GguCCAUcu -5' |
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| 30457 | 5' | -56.4 | NC_006548.1 | + | 33278 | 0.66 | 0.50976 |
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Target: 5'- cGGCAGCCcGGuuucGUUAUCCAGGa--- -3' miRNA: 3'- -UCGUCGGaCCca--CGAUAGGUCCaucu -5' |
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| 30457 | 5' | -56.4 | NC_006548.1 | + | 23759 | 0.67 | 0.426683 |
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Target: 5'- cAGCAGCCUauGUGCUGcuggcCCAGG-AGAc -3' miRNA: 3'- -UCGUCGGAccCACGAUa----GGUCCaUCU- -5' |
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| 30457 | 5' | -56.4 | NC_006548.1 | + | 22918 | 0.68 | 0.379018 |
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Target: 5'- cGGCGGCgagUUGGGccgGaCUAUCgGGGUGGAc -3' miRNA: 3'- -UCGUCG---GACCCa--C-GAUAGgUCCAUCU- -5' |
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| 30457 | 5' | -56.4 | NC_006548.1 | + | 23100 | 1.09 | 0.000394 |
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Target: 5'- gAGCAGCCUGGGUGCUAUCCAGGUAGAu -3' miRNA: 3'- -UCGUCGGACCCACGAUAGGUCCAUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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