Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30460 | 5' | -56.5 | NC_006548.1 | + | 27029 | 0.66 | 0.551025 |
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Target: 5'- aCGGcCCGcAUCAGGGCCaugGUG-GCGg -3' miRNA: 3'- gGUC-GGC-UAGUCCCGGaa-CGCaUGCa -5' |
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| 30460 | 5' | -56.5 | NC_006548.1 | + | 2442 | 0.66 | 0.517494 |
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Target: 5'- uCCAGCCGGcUCGGauggaaaaccgccGGCCUggcGgGUGCGc -3' miRNA: 3'- -GGUCGGCU-AGUC-------------CCGGAa--CgCAUGCa -5' |
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| 30460 | 5' | -56.5 | NC_006548.1 | + | 15860 | 0.67 | 0.486886 |
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Target: 5'- cCCAGCCGAUacccaGGCCggugGCGU-CGa -3' miRNA: 3'- -GGUCGGCUAguc--CCGGaa--CGCAuGCa -5' |
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| 30460 | 5' | -56.5 | NC_006548.1 | + | 24359 | 0.68 | 0.446143 |
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Target: 5'- -aGGCCGGUCuGGGCCggaaaCGUGCc- -3' miRNA: 3'- ggUCGGCUAGuCCCGGaac--GCAUGca -5' |
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| 30460 | 5' | -56.5 | NC_006548.1 | + | 14526 | 0.69 | 0.388796 |
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Target: 5'- --cGCCG-UCAGcGCCUcgcUGCGUGCGUc -3' miRNA: 3'- gguCGGCuAGUCcCGGA---ACGCAUGCA- -5' |
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| 30460 | 5' | -56.5 | NC_006548.1 | + | 33858 | 0.71 | 0.282215 |
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Target: 5'- uCCAGCgGAUC-GGGCCgagGCGgaaguCGUa -3' miRNA: 3'- -GGUCGgCUAGuCCCGGaa-CGCau---GCA- -5' |
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| 30460 | 5' | -56.5 | NC_006548.1 | + | 23996 | 1.08 | 0.000559 |
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Target: 5'- uCCAGCCGAUCAGGGCCUUGCGUACGUc -3' miRNA: 3'- -GGUCGGCUAGUCCCGGAACGCAUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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