Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30462 | 5' | -57.5 | NC_006548.1 | + | 18711 | 0.66 | 0.493573 |
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Target: 5'- cGGCGGUug---UCGCCauggcuuucGCAUCGCGCa -3' miRNA: 3'- uCCGCCAcuguaGGCGG---------UGUAGCGUG- -5' |
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| 30462 | 5' | -57.5 | NC_006548.1 | + | 21204 | 0.66 | 0.493573 |
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Target: 5'- cGGGCGGcGAUGg-CGCCGC-UgGCACg -3' miRNA: 3'- -UCCGCCaCUGUagGCGGUGuAgCGUG- -5' |
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| 30462 | 5' | -57.5 | NC_006548.1 | + | 9318 | 0.66 | 0.462585 |
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Target: 5'- cGGGCGauauGUGGCAaaUGCCcCAUCGCGa -3' miRNA: 3'- -UCCGC----CACUGUagGCGGuGUAGCGUg -5' |
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| 30462 | 5' | -57.5 | NC_006548.1 | + | 25008 | 0.67 | 0.41333 |
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Target: 5'- ---aGGUGGCuUCgGUCGCAUCGUGCa -3' miRNA: 3'- uccgCCACUGuAGgCGGUGUAGCGUG- -5' |
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| 30462 | 5' | -57.5 | NC_006548.1 | + | 34336 | 0.67 | 0.41333 |
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Target: 5'- cAGGCGuUGGggUAUCCGCCA-AUCGCGu -3' miRNA: 3'- -UCCGCcACU--GUAGGCGGUgUAGCGUg -5' |
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| 30462 | 5' | -57.5 | NC_006548.1 | + | 23894 | 0.67 | 0.412378 |
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Target: 5'- uGGCGaaGUGcACGUuuGCCAUcugccggGUCGCGCc -3' miRNA: 3'- uCCGC--CAC-UGUAggCGGUG-------UAGCGUG- -5' |
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| 30462 | 5' | -57.5 | NC_006548.1 | + | 25433 | 0.68 | 0.376336 |
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Target: 5'- cGGCgaGGUGAUGgaccggaUCGCCACAgaUCGCGCc -3' miRNA: 3'- uCCG--CCACUGUa------GGCGGUGU--AGCGUG- -5' |
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| 30462 | 5' | -57.5 | NC_006548.1 | + | 23551 | 0.7 | 0.278661 |
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Target: 5'- cAGGCGGUGGgGuUCCGCagguacuggcgccCGC-UCGCACc -3' miRNA: 3'- -UCCGCCACUgU-AGGCG-------------GUGuAGCGUG- -5' |
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| 30462 | 5' | -57.5 | NC_006548.1 | + | 25910 | 1.1 | 0.000298 |
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Target: 5'- cAGGCGGUGACAUCCGCCACAUCGCACg -3' miRNA: 3'- -UCCGCCACUGUAGGCGGUGUAGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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