Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30463 | 5' | -55.6 | NC_006548.1 | + | 32802 | 0.66 | 0.580156 |
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Target: 5'- cGCCCCAgCCUGC--AAGGCU-GAgGUc -3' miRNA: 3'- -CGGGGU-GGACGcuUUCCGAgCUgUAu -5' |
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| 30463 | 5' | -55.6 | NC_006548.1 | + | 23544 | 0.66 | 0.535511 |
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Target: 5'- aCCCCGCCagGCGGuGGGGUUCcGCAg- -3' miRNA: 3'- cGGGGUGGa-CGCU-UUCCGAGcUGUau -5' |
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| 30463 | 5' | -55.6 | NC_006548.1 | + | 17032 | 0.67 | 0.502831 |
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Target: 5'- aGCUCCGCCUGCaugccGGCg-GGCAUGa -3' miRNA: 3'- -CGGGGUGGACGcuuu-CCGagCUGUAU- -5' |
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| 30463 | 5' | -55.6 | NC_006548.1 | + | 26518 | 0.67 | 0.47105 |
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Target: 5'- aGUCCUGCCaGCuGAGGuGCUCGAUGUAg -3' miRNA: 3'- -CGGGGUGGaCGcUUUC-CGAGCUGUAU- -5' |
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| 30463 | 5' | -55.6 | NC_006548.1 | + | 4297 | 0.69 | 0.410765 |
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Target: 5'- aCCCgCGcCCUGCGcAAGGC-CGGCAUu -3' miRNA: 3'- cGGG-GU-GGACGCuUUCCGaGCUGUAu -5' |
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| 30463 | 5' | -55.6 | NC_006548.1 | + | 9336 | 0.69 | 0.363454 |
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Target: 5'- uGCCCCAUC-GCGAucuggcaGGGGUgcugCGGCAUGu -3' miRNA: 3'- -CGGGGUGGaCGCU-------UUCCGa---GCUGUAU- -5' |
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| 30463 | 5' | -55.6 | NC_006548.1 | + | 20146 | 0.7 | 0.338288 |
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Target: 5'- gGCaCCACCUG-GugcGGCUCGGCAUAg -3' miRNA: 3'- -CGgGGUGGACgCuuuCCGAGCUGUAU- -5' |
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| 30463 | 5' | -55.6 | NC_006548.1 | + | 28125 | 0.71 | 0.313612 |
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Target: 5'- cGCCagCCGCCUGCuguGAGGCUgGAcCAUAg -3' miRNA: 3'- -CGG--GGUGGACGcu-UUCCGAgCU-GUAU- -5' |
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| 30463 | 5' | -55.6 | NC_006548.1 | + | 27782 | 0.71 | 0.297932 |
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Target: 5'- gGCCgCCACCagGCGGGaauAGGCUUGGCu-- -3' miRNA: 3'- -CGG-GGUGGa-CGCUU---UCCGAGCUGuau -5' |
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| 30463 | 5' | -55.6 | NC_006548.1 | + | 26143 | 1.09 | 0.000485 |
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Target: 5'- cGCCCCACCUGCGAAAGGCUCGACAUAg -3' miRNA: 3'- -CGGGGUGGACGCUUUCCGAGCUGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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