Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30464 | 3' | -52.1 | NC_006548.1 | + | 28190 | 0.66 | 0.793956 |
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Target: 5'- aCCUGCGuGaUgUGCUGAaAGAUCCg- -3' miRNA: 3'- -GGACGUcCaAgACGACUaUCUAGGgu -5' |
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| 30464 | 3' | -52.1 | NC_006548.1 | + | 30557 | 0.66 | 0.773021 |
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Target: 5'- gCC-GCAGGUUCUgGCUGGUcgucaAGGUCagCAc -3' miRNA: 3'- -GGaCGUCCAAGA-CGACUA-----UCUAGg-GU- -5' |
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| 30464 | 3' | -52.1 | NC_006548.1 | + | 30782 | 0.66 | 0.773021 |
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Target: 5'- --cGUAGGggCUGCUGAUGc--CCCGg -3' miRNA: 3'- ggaCGUCCaaGACGACUAUcuaGGGU- -5' |
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| 30464 | 3' | -52.1 | NC_006548.1 | + | 7683 | 0.66 | 0.751438 |
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Target: 5'- uUCUGCAGGUUCUcGgUGGUguuggccagcaGGcgCCCc -3' miRNA: 3'- -GGACGUCCAAGA-CgACUA-----------UCuaGGGu -5' |
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| 30464 | 3' | -52.1 | NC_006548.1 | + | 27077 | 0.66 | 0.751438 |
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Target: 5'- -aUGCGGGccgUCUGCUGAUcggcGGAcUCgCCGa -3' miRNA: 3'- ggACGUCCa--AGACGACUA----UCU-AG-GGU- -5' |
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| 30464 | 3' | -52.1 | NC_006548.1 | + | 25681 | 0.68 | 0.660761 |
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Target: 5'- gCUGCAGGaUCUGCUGAaguaaggaAGggCaCCAg -3' miRNA: 3'- gGACGUCCaAGACGACUa-------UCuaG-GGU- -5' |
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| 30464 | 3' | -52.1 | NC_006548.1 | + | 22109 | 0.69 | 0.602684 |
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Target: 5'- uCCUGCAGGagCUGCUGGaacUGGA-CgCGa -3' miRNA: 3'- -GGACGUCCaaGACGACU---AUCUaGgGU- -5' |
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| 30464 | 3' | -52.1 | NC_006548.1 | + | 961 | 0.75 | 0.300657 |
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Target: 5'- aCCUGCAGcUUCUGCcGAUAGAcaucgUCCUg -3' miRNA: 3'- -GGACGUCcAAGACGaCUAUCU-----AGGGu -5' |
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| 30464 | 3' | -52.1 | NC_006548.1 | + | 26814 | 1.11 | 0.000854 |
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Target: 5'- gCCUGCAGGUUCUGCUGAUAGAUCCCAg -3' miRNA: 3'- -GGACGUCCAAGACGACUAUCUAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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