Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30465 | 3' | -58.8 | NC_006548.1 | + | 5925 | 0.66 | 0.420309 |
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Target: 5'- --uUGCGGGCCuUCUGCgccggagucucagccUGA-CGGCu -3' miRNA: 3'- acuACGCCCGGcAGACG---------------ACUaGCCGc -5' |
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| 30465 | 3' | -58.8 | NC_006548.1 | + | 29362 | 0.66 | 0.416518 |
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Target: 5'- gGAUcGCaGaGCCaaaGUgaGCUGGUCGGCGg -3' miRNA: 3'- aCUA-CGcC-CGG---CAgaCGACUAGCCGC- -5' |
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| 30465 | 3' | -58.8 | NC_006548.1 | + | 13415 | 0.67 | 0.37979 |
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Target: 5'- gGcgGCGGGCCagGUC-GCUGuuugagcgcCGGCGg -3' miRNA: 3'- aCuaCGCCCGG--CAGaCGACua-------GCCGC- -5' |
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| 30465 | 3' | -58.8 | NC_006548.1 | + | 2310 | 0.68 | 0.328909 |
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Target: 5'- cGcgGCGGcGCCGga-GCUGAcCGGCu -3' miRNA: 3'- aCuaCGCC-CGGCagaCGACUaGCCGc -5' |
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| 30465 | 3' | -58.8 | NC_006548.1 | + | 16328 | 0.68 | 0.297885 |
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Target: 5'- gUGAUGuCGcGGCCGUCgccaCUGGU-GGCGa -3' miRNA: 3'- -ACUAC-GC-CCGGCAGac--GACUAgCCGC- -5' |
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| 30465 | 3' | -58.8 | NC_006548.1 | + | 26977 | 0.69 | 0.290491 |
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Target: 5'- cGAgcaaGCuGGCCGUaaGCUGAgcagcgUCGGCGa -3' miRNA: 3'- aCUa---CGcCCGGCAgaCGACU------AGCCGC- -5' |
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| 30465 | 3' | -58.8 | NC_006548.1 | + | 21129 | 0.69 | 0.283243 |
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Target: 5'- gUGAUGUGGGUaacggccucgGUCgUGCUGAUaUGGCGu -3' miRNA: 3'- -ACUACGCCCGg---------CAG-ACGACUA-GCCGC- -5' |
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| 30465 | 3' | -58.8 | NC_006548.1 | + | 32871 | 0.69 | 0.276139 |
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Target: 5'- gUGcUGgGGGCCuugcgCUGCUGcgCGGUGa -3' miRNA: 3'- -ACuACgCCCGGca---GACGACuaGCCGC- -5' |
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| 30465 | 3' | -58.8 | NC_006548.1 | + | 27080 | 1.09 | 0.000268 |
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Target: 5'- cUGAUGCGGGCCGUCUGCUGAUCGGCGg -3' miRNA: 3'- -ACUACGCCCGGCAGACGACUAGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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