Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30465 | 5' | -57 | NC_006548.1 | + | 7163 | 0.66 | 0.455842 |
Target: 5'- cGCGCUcacGCaccuguacauccuUCAGCUUGCGGUaCAGCg- -3' miRNA: 3'- cUGCGA---CG-------------AGUCGAAUGCCG-GUCGaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 20911 | 0.66 | 0.446584 |
Target: 5'- -gUGCUGCgCAGCUUGCuGGCCGa--- -3' miRNA: 3'- cuGCGACGaGUCGAAUG-CCGGUcgaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 37102 | 0.66 | 0.426392 |
Target: 5'- -gUGCUGUUCAGCguucgcgACGGUCuGCa- -3' miRNA: 3'- cuGCGACGAGUCGaa-----UGCCGGuCGaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 9177 | 0.66 | 0.426392 |
Target: 5'- uGACGCUGUggcaaCAGCUUugccaACGGUgaaaaCAGCUa -3' miRNA: 3'- -CUGCGACGa----GUCGAA-----UGCCG-----GUCGAa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 32802 | 0.66 | 0.416502 |
Target: 5'- --aGCUGCUggcaAGCgucaccGCGGCCGGCg- -3' miRNA: 3'- cugCGACGAg---UCGaa----UGCCGGUCGaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 32386 | 0.66 | 0.416502 |
Target: 5'- gGACGCcgaugGCaUCAGCcuggcCGGCCAGCc- -3' miRNA: 3'- -CUGCGa----CG-AGUCGaau--GCCGGUCGaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 1191 | 0.66 | 0.416502 |
Target: 5'- aGGCGUUGCUgGccGCgcagACGGCCAGgCUc -3' miRNA: 3'- -CUGCGACGAgU--CGaa--UGCCGGUC-GAa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 12583 | 0.66 | 0.416502 |
Target: 5'- --aGCUGgUCGGCgccggaUGCcgaGGCCAGCUUa -3' miRNA: 3'- cugCGACgAGUCGa-----AUG---CCGGUCGAA- -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 12085 | 0.67 | 0.406753 |
Target: 5'- uGCGCUgauGCUCcuGCUgcUGGCCGGCg- -3' miRNA: 3'- cUGCGA---CGAGu-CGAauGCCGGUCGaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 29906 | 0.67 | 0.397149 |
Target: 5'- cGGCGCUGUgCGGCacgcgACGGgCGGCa- -3' miRNA: 3'- -CUGCGACGaGUCGaa---UGCCgGUCGaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 9521 | 0.68 | 0.342679 |
Target: 5'- gGAgGCUGCUCugguGCgcguCGGCCAGgUg -3' miRNA: 3'- -CUgCGACGAGu---CGaau-GCCGGUCgAa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 16542 | 0.68 | 0.342679 |
Target: 5'- aGAUGCUGUUCGGCguguucUACGGUUAcGCg- -3' miRNA: 3'- -CUGCGACGAGUCGa-----AUGCCGGU-CGaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 20850 | 0.68 | 0.334141 |
Target: 5'- uGGCGCgg--CAGCagGCGGCCGGCc- -3' miRNA: 3'- -CUGCGacgaGUCGaaUGCCGGUCGaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 22817 | 0.68 | 0.32576 |
Target: 5'- -gUGCUGCUCAGCg-ACGGUUcGCUg -3' miRNA: 3'- cuGCGACGAGUCGaaUGCCGGuCGAa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 29487 | 0.68 | 0.309469 |
Target: 5'- -cUGCUGCgCGGCaacguCGGCCAGCg- -3' miRNA: 3'- cuGCGACGaGUCGaau--GCCGGUCGaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 28527 | 0.7 | 0.230955 |
Target: 5'- uGACGCaguccUGCUCGGCgc-CGGCCAGg-- -3' miRNA: 3'- -CUGCG-----ACGAGUCGaauGCCGGUCgaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 17534 | 0.71 | 0.224718 |
Target: 5'- cGACagGCUGCaUC-GCUU-CGGCCAGCUg -3' miRNA: 3'- -CUG--CGACG-AGuCGAAuGCCGGUCGAa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 14668 | 0.71 | 0.224718 |
Target: 5'- aGGCGCUGCUC-GCUgagcagaaGGCCAaGCUg -3' miRNA: 3'- -CUGCGACGAGuCGAaug-----CCGGU-CGAa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 25633 | 0.71 | 0.206862 |
Target: 5'- cGACGCUGUUCGGCUUcAUGGagCAGUa- -3' miRNA: 3'- -CUGCGACGAGUCGAA-UGCCg-GUCGaa -5' |
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30465 | 5' | -57 | NC_006548.1 | + | 33317 | 0.71 | 0.205145 |
Target: 5'- cGGCGCUGCgCAGCcUGCuguacgcccugcguGGCCGGCa- -3' miRNA: 3'- -CUGCGACGaGUCGaAUG--------------CCGGUCGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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