Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30466 | 3' | -56.7 | NC_006548.1 | + | 28541 | 0.66 | 0.512469 |
Target: 5'- uCUGCGCUugugccuugcUGAGUGCUucuUCGUaucGCUGGa -3' miRNA: 3'- uGACGCGA----------ACUCGCGAu--AGCG---CGGUC- -5' |
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30466 | 3' | -56.7 | NC_006548.1 | + | 15825 | 0.66 | 0.512469 |
Target: 5'- -aUGCGCUcacAGCGCUGgaaccaGgGCCAGa -3' miRNA: 3'- ugACGCGAac-UCGCGAUag----CgCGGUC- -5' |
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30466 | 3' | -56.7 | NC_006548.1 | + | 30791 | 0.66 | 0.491064 |
Target: 5'- aGCUGCGCUcguagGGGCuGCUGaUGCcCCGGu -3' miRNA: 3'- -UGACGCGAa----CUCG-CGAUaGCGcGGUC- -5' |
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30466 | 3' | -56.7 | NC_006548.1 | + | 34867 | 0.66 | 0.480517 |
Target: 5'- cACUGCGCagauGCGCUGUUGUGaugauCAGa -3' miRNA: 3'- -UGACGCGaacuCGCGAUAGCGCg----GUC- -5' |
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30466 | 3' | -56.7 | NC_006548.1 | + | 6927 | 0.66 | 0.470081 |
Target: 5'- aGCUGUGC---AGCGCgcggcugAUCGCGCUg- -3' miRNA: 3'- -UGACGCGaacUCGCGa------UAGCGCGGuc -5' |
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30466 | 3' | -56.7 | NC_006548.1 | + | 15773 | 0.68 | 0.363867 |
Target: 5'- --aGCGCUgUGAGCGCaucugcAUCGaCGCCAc -3' miRNA: 3'- ugaCGCGA-ACUCGCGa-----UAGC-GCGGUc -5' |
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30466 | 3' | -56.7 | NC_006548.1 | + | 8031 | 0.69 | 0.321391 |
Target: 5'- cACUGaacaGUUggggauacaUGAGCGCgcagCGCGCCGGa -3' miRNA: 3'- -UGACg---CGA---------ACUCGCGaua-GCGCGGUC- -5' |
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30466 | 3' | -56.7 | NC_006548.1 | + | 8587 | 0.71 | 0.254533 |
Target: 5'- cGCUGCGCguccuGCGCguucugAUCGCGCUg- -3' miRNA: 3'- -UGACGCGaacu-CGCGa-----UAGCGCGGuc -5' |
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30466 | 3' | -56.7 | NC_006548.1 | + | 20655 | 0.71 | 0.247854 |
Target: 5'- aGCUGCGCUccagGAGCGCcaa--CGCCAGc -3' miRNA: 3'- -UGACGCGAa---CUCGCGauagcGCGGUC- -5' |
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30466 | 3' | -56.7 | NC_006548.1 | + | 9592 | 0.72 | 0.199527 |
Target: 5'- --aGUGCaucGAGCGCgg-CGCGCCAGg -3' miRNA: 3'- ugaCGCGaa-CUCGCGauaGCGCGGUC- -5' |
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30466 | 3' | -56.7 | NC_006548.1 | + | 27206 | 1.09 | 0.000364 |
Target: 5'- gACUGCGCUUGAGCGCUAUCGCGCCAGg -3' miRNA: 3'- -UGACGCGAACUCGCGAUAGCGCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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