Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30468 | 3' | -57.5 | NC_006548.1 | + | 7994 | 0.66 | 0.477167 |
Target: 5'- -cGGAUGAUGCagGCGGCgcucaaauaccuguCUCCUGCAc -3' miRNA: 3'- ucCUUGCUAUGg-CGCCG--------------GAGGACGUc -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 14287 | 0.66 | 0.469988 |
Target: 5'- -uGGACGAgcugAUCGCGGa--CCUGCAGg -3' miRNA: 3'- ucCUUGCUa---UGGCGCCggaGGACGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 28108 | 0.67 | 0.43987 |
Target: 5'- gAGGc-UGG-ACCauaGGCCUCCUGCAGg -3' miRNA: 3'- -UCCuuGCUaUGGcg-CCGGAGGACGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 27040 | 0.67 | 0.430077 |
Target: 5'- cAGGGccAUGGUG--GCGGCCUCgCUGCAa -3' miRNA: 3'- -UCCU--UGCUAUggCGCCGGAG-GACGUc -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 22942 | 0.67 | 0.414678 |
Target: 5'- cGGGGugGAcugGCCGcCGGCCucaaggaccgcuucaUCgUGCAGc -3' miRNA: 3'- -UCCUugCUa--UGGC-GCCGG---------------AGgACGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 3450 | 0.68 | 0.392228 |
Target: 5'- gAGGAGCagcGCCGUGGCCUUCacCAGg -3' miRNA: 3'- -UCCUUGcuaUGGCGCCGGAGGacGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 18448 | 0.68 | 0.392228 |
Target: 5'- cGGGugGcaGCggCGUGGUCUUCUGCAGg -3' miRNA: 3'- uCCUugCuaUG--GCGCCGGAGGACGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 11593 | 0.68 | 0.3653 |
Target: 5'- --cGAUGAUGuCCG-GGCgUCCUGCAGg -3' miRNA: 3'- uccUUGCUAU-GGCgCCGgAGGACGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 20024 | 0.7 | 0.292423 |
Target: 5'- cGGAuCGuagGUCGCGGCgauCUCCUGCAGg -3' miRNA: 3'- uCCUuGCua-UGGCGCCG---GAGGACGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 26337 | 0.7 | 0.270791 |
Target: 5'- uGGAuguCGGUcagcguuuccgGCCG-GGUCUCCUGCAGc -3' miRNA: 3'- uCCUu--GCUA-----------UGGCgCCGGAGGACGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 29385 | 0.7 | 0.263872 |
Target: 5'- cAGGAG----GCCGCGGUaucgUUCCUGCAGg -3' miRNA: 3'- -UCCUUgcuaUGGCGCCG----GAGGACGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 3868 | 0.7 | 0.257097 |
Target: 5'- cGGAGCugcaGUGCaugGCGGUCUaCCUGCAGg -3' miRNA: 3'- uCCUUGc---UAUGg--CGCCGGA-GGACGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 20610 | 0.71 | 0.250465 |
Target: 5'- gGGGuGACGGUGCCcuGCGGCuCUUCcGCAGc -3' miRNA: 3'- -UCC-UUGCUAUGG--CGCCG-GAGGaCGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 22087 | 0.81 | 0.045564 |
Target: 5'- gAGGGcAUGGUGCCGaUGGCCuUCCUGCAGg -3' miRNA: 3'- -UCCU-UGCUAUGGC-GCCGG-AGGACGUC- -5' |
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30468 | 3' | -57.5 | NC_006548.1 | + | 29446 | 1.08 | 0.000462 |
Target: 5'- cAGGAACGAUACCGCGGCCUCCUGCAGc -3' miRNA: 3'- -UCCUUGCUAUGGCGCCGGAGGACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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