Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30470 | 5' | -61.4 | NC_006548.1 | + | 33117 | 0.66 | 0.306108 |
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Target: 5'- gGUCAUGCCCucgacaacauaccggGGCUggccggugcucGGGcaGCGGCC-Cg -3' miRNA: 3'- -CAGUACGGG---------------CCGA-----------CCCaaCGCCGGcG- -5' |
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| 30470 | 5' | -61.4 | NC_006548.1 | + | 37465 | 0.67 | 0.247215 |
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Target: 5'- -aCAUGCCgccaCGGCUGGGcugGCGuguccaaGUCGCa -3' miRNA: 3'- caGUACGG----GCCGACCCaa-CGC-------CGGCG- -5' |
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| 30470 | 5' | -61.4 | NC_006548.1 | + | 30833 | 0.69 | 0.195929 |
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Target: 5'- aUCGgacGCUCcucccGCUGGGcUGCGGCCGUu -3' miRNA: 3'- cAGUa--CGGGc----CGACCCaACGCCGGCG- -5' |
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| 30470 | 5' | -61.4 | NC_006548.1 | + | 27808 | 0.7 | 0.162362 |
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Target: 5'- ---cUGCCCaGCUccgGGGUUucuuucGCGGCCGCc -3' miRNA: 3'- caguACGGGcCGA---CCCAA------CGCCGGCG- -5' |
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| 30470 | 5' | -61.4 | NC_006548.1 | + | 35948 | 0.71 | 0.137807 |
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Target: 5'- ---uUGgCCGGCaGGGUgGCGGCgCGCg -3' miRNA: 3'- caguACgGGCCGaCCCAaCGCCG-GCG- -5' |
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| 30470 | 5' | -61.4 | NC_006548.1 | + | 11752 | 0.75 | 0.073026 |
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Target: 5'- gGUCAUGCgCGGCgcgcggcggcgccgGGGUUGCGGCg-- -3' miRNA: 3'- -CAGUACGgGCCGa-------------CCCAACGCCGgcg -5' |
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| 30470 | 5' | -61.4 | NC_006548.1 | + | 29889 | 1.09 | 0.000135 |
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Target: 5'- gGUCAUGCCCGGCUGGGUUGCGGCCGCc -3' miRNA: 3'- -CAGUACGGGCCGACCCAACGCCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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