Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30473 | 3' | -54.5 | NC_006548.1 | + | 31314 | 1.11 | 0.000537 |
Target: 5'- uGGCCCCGGCACUGAAUGACCAGUAUCc -3' miRNA: 3'- -CCGGGGCCGUGACUUACUGGUCAUAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 17868 | 0.82 | 0.06157 |
Target: 5'- uGCCCCGGCGCUGAcgaagGACCGGUcGUUa -3' miRNA: 3'- cCGGGGCCGUGACUua---CUGGUCA-UAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 23387 | 0.72 | 0.310524 |
Target: 5'- aGCCCCGGCGCUGGAggaaGCC---GUCg -3' miRNA: 3'- cCGGGGCCGUGACUUac--UGGucaUAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 36520 | 0.72 | 0.326725 |
Target: 5'- aGGCCCCGGCcgauaucuGCUGAGaucgucgcGACCGGgcagaaGUCg -3' miRNA: 3'- -CCGGGGCCG--------UGACUUa-------CUGGUCa-----UAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 2908 | 0.71 | 0.369922 |
Target: 5'- cGGCCaaGGCGCUGAucGACgAGUGc- -3' miRNA: 3'- -CCGGggCCGUGACUuaCUGgUCAUag -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 37774 | 0.7 | 0.426683 |
Target: 5'- cGGUCgCCGGCACUGuGAUGAgC-GUGUUc -3' miRNA: 3'- -CCGG-GGCCGUGAC-UUACUgGuCAUAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 16785 | 0.69 | 0.477728 |
Target: 5'- uGGCCCauuGGCugUac-UGGCCGGUGUUc -3' miRNA: 3'- -CCGGGg--CCGugAcuuACUGGUCAUAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 34877 | 0.68 | 0.536018 |
Target: 5'- aGCCCCGGUacacugcgcagaugcGCUGuuguGAUGAUCAG-AUCc -3' miRNA: 3'- cCGGGGCCG---------------UGAC----UUACUGGUCaUAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 34138 | 0.67 | 0.553783 |
Target: 5'- uGCaCCCGGCugUcGGccGGCCAGU-UCa -3' miRNA: 3'- cCG-GGGCCGugA-CUuaCUGGUCAuAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 22962 | 0.67 | 0.587525 |
Target: 5'- cGCCgCCGcGCgACUGAAUcGCCAGU-UCg -3' miRNA: 3'- cCGG-GGC-CG-UGACUUAcUGGUCAuAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 28231 | 0.67 | 0.598864 |
Target: 5'- aGGUCUcgcaCGGC-CUGAGUGAa-GGUGUCg -3' miRNA: 3'- -CCGGG----GCCGuGACUUACUggUCAUAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 36693 | 0.67 | 0.598864 |
Target: 5'- cGGCCUcaugCGGCGCUGGc--ACC-GUAUCg -3' miRNA: 3'- -CCGGG----GCCGUGACUuacUGGuCAUAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 19597 | 0.67 | 0.598864 |
Target: 5'- uGGCCgagCCGGCAuCUGGAUuGugUAGgGUCc -3' miRNA: 3'- -CCGG---GGCCGU-GACUUA-CugGUCaUAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 32965 | 0.66 | 0.631885 |
Target: 5'- cGCCCggugcagCGGCGCUGGccGGCCGGc--- -3' miRNA: 3'- cCGGG-------GCCGUGACUuaCUGGUCauag -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 17436 | 0.66 | 0.641006 |
Target: 5'- uGGCCUCGGC-CUGggUGGacucaacuucgaccCCAuagGUCu -3' miRNA: 3'- -CCGGGGCCGuGACuuACU--------------GGUca-UAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 6889 | 0.66 | 0.644426 |
Target: 5'- cGGCUCCGGCGCcaauagcugggaUGGAUGcaGgCGGUAg- -3' miRNA: 3'- -CCGGGGCCGUG------------ACUUAC--UgGUCAUag -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 25079 | 0.66 | 0.666042 |
Target: 5'- cGCCCCGGCcaccgauccagggGC-GGAUGACCuGcagAUCc -3' miRNA: 3'- cCGGGGCCG-------------UGaCUUACUGGuCa--UAG- -5' |
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30473 | 3' | -54.5 | NC_006548.1 | + | 31211 | 0.66 | 0.667177 |
Target: 5'- gGGCUUCGGCAaccGAGUGGCUc-UGUCa -3' miRNA: 3'- -CCGGGGCCGUga-CUUACUGGucAUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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