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miRNA: 3'- aagAUGAGCUGG-UGGGCCUUacGUAU- -5'
miRNA: 3'- aaGAUGAgCUGGUGGGC-CUUaCGUau -5'
miRNA: 3'- -AAGAUGAGCUGGUGGGCCUUACGUAU- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 30476 | 5' | -52.9 | NC_006548.1 | + | 11877 | 0.66 | 0.676018 |
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Target: 5'- --aUGCUCGGCCaACCUGGAGguCGUAg -3' miRNA: 3'- aagAUGAGCUGG-UGGGCCUUacGUAU- -5' |
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| 30476 | 5' | -52.9 | NC_006548.1 | + | 20700 | 0.67 | 0.640986 |
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Target: 5'- -aCUGCU-GGCCACCCGcGAA-GCAc- -3' miRNA: 3'- aaGAUGAgCUGGUGGGC-CUUaCGUau -5' |
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| 30476 | 5' | -52.9 | NC_006548.1 | + | 32083 | 1.05 | 0.001701 |
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Target: 5'- uUUCUACUCGACCACCCGGAAUGCAUAg -3' miRNA: 3'- -AAGAUGAGCUGGUGGGCCUUACGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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