Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30477 | 3' | -55.4 | NC_006548.1 | + | 30582 | 0.66 | 0.62839 |
Target: 5'- gGUCAGcacgauuuacGAGUCgGCCGGCGCGGgcuUCAu -3' miRNA: 3'- gCGGUCaa--------CUUAG-CGGCUGCGCC---AGU- -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 37527 | 0.66 | 0.617161 |
Target: 5'- aCGCCAGccca---GCCGugGCGG-CAu -3' miRNA: 3'- -GCGGUCaacuuagCGGCugCGCCaGU- -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 17142 | 0.66 | 0.617161 |
Target: 5'- uCGCCuugaccuggUGAAg-GCCGACGCGGaUCu -3' miRNA: 3'- -GCGGuca------ACUUagCGGCUGCGCC-AGu -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 20105 | 0.67 | 0.572481 |
Target: 5'- uGCCGGagu--UCGCCGAgGCGG-CGa -3' miRNA: 3'- gCGGUCaacuuAGCGGCUgCGCCaGU- -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 22756 | 0.67 | 0.526443 |
Target: 5'- gGCCAGUcccgaagcggcgGAGUCGCCuuUGCGGaUCGc -3' miRNA: 3'- gCGGUCAa-----------CUUAGCGGcuGCGCC-AGU- -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 36779 | 0.67 | 0.525362 |
Target: 5'- gCGCCGGUcgacucgcugcgaaUGAAccagCGCCaGGCGCGGcCu -3' miRNA: 3'- -GCGGUCA--------------ACUUa---GCGG-CUGCGCCaGu -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 23617 | 0.68 | 0.496531 |
Target: 5'- gCGCCAGUaccugcgGAAccccacCGCCuGGCGgGGUCAg -3' miRNA: 3'- -GCGGUCAa------CUUa-----GCGG-CUGCgCCAGU- -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 12814 | 0.68 | 0.465364 |
Target: 5'- uCGCCAGUgccg-CGCgagCGGCGCGGUa- -3' miRNA: 3'- -GCGGUCAacuuaGCG---GCUGCGCCAgu -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 8094 | 0.68 | 0.465364 |
Target: 5'- gCGCCucaUUGAccaCGCgGAUGCGGUCGu -3' miRNA: 3'- -GCGGuc-AACUua-GCGgCUGCGCCAGU- -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 36952 | 0.7 | 0.406281 |
Target: 5'- aGCCGGUUGcggugCGCCcacGACaCGGUCAg -3' miRNA: 3'- gCGGUCAACuua--GCGG---CUGcGCCAGU- -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 33440 | 0.7 | 0.3969 |
Target: 5'- aGCUGGUUGG---GCUGGCGCGGUUc -3' miRNA: 3'- gCGGUCAACUuagCGGCUGCGCCAGu -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 1127 | 0.7 | 0.387659 |
Target: 5'- gCGCCGGcUGggUCGUCGACaUGG-CAu -3' miRNA: 3'- -GCGGUCaACuuAGCGGCUGcGCCaGU- -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 32326 | 0.7 | 0.369607 |
Target: 5'- gGCCAG--GAAUCaGCCaGAUGCGGUUg -3' miRNA: 3'- gCGGUCaaCUUAG-CGG-CUGCGCCAGu -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 36635 | 0.7 | 0.360799 |
Target: 5'- gGCCGcggcGUUGAucgCGUCGACGCGGa-- -3' miRNA: 3'- gCGGU----CAACUua-GCGGCUGCGCCagu -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 7097 | 0.71 | 0.335263 |
Target: 5'- gCGCCuGUauuccGUUGCUGAUGCGGUCGa -3' miRNA: 3'- -GCGGuCAacu--UAGCGGCUGCGCCAGU- -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 1464 | 0.71 | 0.32705 |
Target: 5'- gCGCCgAGgcGGAUCGCCGGCaGUGGa-- -3' miRNA: 3'- -GCGG-UCaaCUUAGCGGCUG-CGCCagu -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 8294 | 0.72 | 0.288233 |
Target: 5'- cCGgCGGUUGAuuUCGuuGAUGCGGUUc -3' miRNA: 3'- -GCgGUCAACUu-AGCggCUGCGCCAGu -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 18456 | 0.72 | 0.273752 |
Target: 5'- aGCCGGUUcGggUgGCagCGGCGUGGUCu -3' miRNA: 3'- gCGGUCAA-CuuAgCG--GCUGCGCCAGu -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 870 | 0.73 | 0.253131 |
Target: 5'- aGCCAGUUGcc-CGCCGAgGUccaGGUCAu -3' miRNA: 3'- gCGGUCAACuuaGCGGCUgCG---CCAGU- -5' |
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30477 | 3' | -55.4 | NC_006548.1 | + | 33003 | 1.09 | 0.00067 |
Target: 5'- gCGCCAGUUGAAUCGCCGACGCGGUCAg -3' miRNA: 3'- -GCGGUCAACUUAGCGGCUGCGCCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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