Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30477 | 5' | -51.3 | NC_006548.1 | + | 4139 | 0.66 | 0.841679 |
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Target: 5'- uCUCGucaGCCugCUCGgcgUCGAuguGgGCUGg -3' miRNA: 3'- -GAGC---UGGugGAGCauaAGCU---UgCGAC- -5' |
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| 30477 | 5' | -51.3 | NC_006548.1 | + | 23708 | 0.67 | 0.813212 |
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Target: 5'- gCUCGAUCACCaggUUGUGaUCGAaagguGCGCa- -3' miRNA: 3'- -GAGCUGGUGG---AGCAUaAGCU-----UGCGac -5' |
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| 30477 | 5' | -51.3 | NC_006548.1 | + | 29334 | 0.67 | 0.793173 |
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Target: 5'- -gCGGCCAgCCggGUAUugUCGAGCuGCUGg -3' miRNA: 3'- gaGCUGGU-GGagCAUA--AGCUUG-CGAC- -5' |
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| 30477 | 5' | -51.3 | NC_006548.1 | + | 37716 | 0.68 | 0.729047 |
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Target: 5'- gUCGACgACCUCGguaUCGAACaCUa -3' miRNA: 3'- gAGCUGgUGGAGCauaAGCUUGcGAc -5' |
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| 30477 | 5' | -51.3 | NC_006548.1 | + | 32078 | 0.69 | 0.699905 |
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Target: 5'- aCUCGACCACC-CGgaaugcauagcCGAccagcGCGCUGg -3' miRNA: 3'- -GAGCUGGUGGaGCauaa-------GCU-----UGCGAC- -5' |
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| 30477 | 5' | -51.3 | NC_006548.1 | + | 28956 | 0.69 | 0.695372 |
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Target: 5'- -cCGGCCGCCUCGacgGUUUGGcCGgUGg -3' miRNA: 3'- gaGCUGGUGGAGCa--UAAGCUuGCgAC- -5' |
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| 30477 | 5' | -51.3 | NC_006548.1 | + | 31573 | 0.69 | 0.672565 |
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Target: 5'- -cUGGCCGCCgcuGUAUUCGAcuccGCGCUu -3' miRNA: 3'- gaGCUGGUGGag-CAUAAGCU----UGCGAc -5' |
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| 30477 | 5' | -51.3 | NC_006548.1 | + | 23647 | 0.71 | 0.569211 |
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Target: 5'- uUCGAUCacaACCUgGUGaUCGAGCGCUu -3' miRNA: 3'- gAGCUGG---UGGAgCAUaAGCUUGCGAc -5' |
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| 30477 | 5' | -51.3 | NC_006548.1 | + | 1517 | 0.74 | 0.409244 |
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Target: 5'- -gCGACCGCCUCGg---CGcGAUGCUGa -3' miRNA: 3'- gaGCUGGUGGAGCauaaGC-UUGCGAC- -5' |
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| 30477 | 5' | -51.3 | NC_006548.1 | + | 32967 | 1.09 | 0.001756 |
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Target: 5'- uCUCGACCACCUCGUAUUCGAACGCUGa -3' miRNA: 3'- -GAGCUGGUGGAGCAUAAGCUUGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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